http://scholars.ntou.edu.tw/handle/123456789/19098
標題: | Portable and Rapid Sequencing Device with Microbial Community-Guided Culture Strategies for Precious Field and Environmental Samples | 作者: | Ho, Ying-Ning Chen, Yu-Ling Liu, Ding-Yang |
關鍵字: | cultivation;functional metagenomics;uncultured microbes;culture;medium prediction;portable and rapid sequencing device;DNA sequencing;bioinformatics;environmental microbiology | 公開日期: | 1-七月-2021 | 出版社: | AMER SOC MICROBIOLOGY | 卷: | 6 | 期: | 4 | 來源出版物: | MSYSTEMS | 摘要: | Culturing unculturable bacteria is a classic microbiology challenge; to successfully culture unculturable bacteria, microbiologists work hard to create hundreds of culture conditions. To improve the success rate and efficiency of culturing a broad spectrum of environmental microbes, it is helpful to know more about the microbial community composition. Shortening the amount of time spent sequencing, analyzing sequencing data, and predicting suitable culture conditions seems to be a critical step for improving knowledge of microbes in environmental samples and expanding culture collections. In this commentary, we introduce potential strategies using rapid and portable sequencing devices to help scientists design and plan for specific microbial culture media on their way back to the laboratory. Rapid metagenomic sequencing approaches and real-time analysis make it possible to choose microbes we are interested in and discover novel microbes in environments for cultivation. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/19098 | ISSN: | 2379-5077 | DOI: | 10.1128/mSystems.00748-21 |
顯示於: | 海洋生物研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。