http://scholars.ntou.edu.tw/handle/123456789/20485
標題: | Molecular cloning and characterization of a sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) from Y-organs of the blue crab (Callinectes sapidus) | 作者: | Megan E. Roegner Hsiang-Yin Chen R. Douglas Watson |
關鍵字: | MOLT-INHIBITING HORMONE;HIGH-DENSITY-LIPOPROTEIN;AMINO-ACID-SEQUENCE;CANCER-ANTENNARIUS;CALCIUM-CONCENTRATION;SIGNALING PATHWAYS;CA2+-ATPASE SERCA;AMERICAN CRAYFISH;EYESTALK REMOVAL;IN-VITRO | 公開日期: | 5-十月-2018 | 出版社: | ELSEVIER | 卷: | 673 | 起(迄)頁: | 12-21 | 來源出版物: | GENE | 摘要: | Existing data indicate that a Ca2+ signal stimulates ecdysteroid hormone production by crustacean molting glands (Y-organs). Ca2+ signaling is dependent on a tightly regulated Ca2+ gradient, with intracellular free Ca2+ maintained at a low basal level (typically sub-micromolar). This is achieved through the action of proteins intrinsic to the plasma membrane and the membranes of organelles. One such protein, the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), pumps Ca2+ from cytosol to the lumen of the endoplasmic reticulum. As a step toward understanding Ca2+-mediated regulation of ecdysteroidogenesis, we have begun investigating Ca2+ transport proteins in Y-organs. In studies reported here, we used a PCR-based strategy to clone from Y-organs of the blue crab (Callinectes sapidus) a cDNA encoding a putative SERCA protein. The cloned Cas-SERCA cDNA (3806 bp) includes a 3057-bp open reading frame that encodes a 1019-residue protein (Cas-SERCA). The conceptually translated protein has a predicted molecular mass of 111.42 x 10(3) and contains all signature domains of an authentic SERCA, including ten transmembrane domains and a phosphorylation site at aspartate 351. A homology model of Cas-SERCA closely resembles models of related SERCA proteins. Phylogenetic analysis shows Cas-SERCA clusters with SERCA proteins from other arthropods. An assessment of tissue distribution indicates the Cas-SERCA transcript is widely distributed across tissues. Studies using quantitative PCR showed Cas-SERCA transcript abundance increased significantly in Y-organs activated by eyestalk ablation, a pattern consistent with the hypothesis that Cas-SERCA functions to maintain Ca2+ homeostasis in Y-organs. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20485 | ISSN: | 0378-1119 | DOI: | 10.1016/j.gene.2018.06.018 |
顯示於: | 03 GOOD HEALTH AND WELL-BEING |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。