http://scholars.ntou.edu.tw/handle/123456789/20540
Title: | Molecular cloning of red pigment-concentrating hormone (RPCH) from eyestalks of the whiteleg shrimp (Litopenaeus vannamei): Evaluation of the effects of the hormone on ovarian growth and the influence of serotonin (5-HT) on its expression | Authors: | Chen, Hsiang-Yin Kang, Bong Jung Sultana, Zakea Wilder, Marcy N. |
Keywords: | VITELLOGENESIS-INHIBITING HORMONE;CRAYFISH PROCAMBARUS-CLARKII;FRESH-WATER PRAWN;CRUSTACEAN HYPERGLYCEMIC HORMONE;MESSENGER-RNA EXPRESSION;SWAMP CRAYFISH;MUD CRAB;DECAPOD CRUSTACEANS;MANDIBULAR ORGAN;IN-VIVO | Issue Date: | 1-Oct-2018 | Publisher: | ELSEVIER | Journal Volume: | 495 | Start page/Pages: | 232-240 | Source: | AQUACULTURE | Abstract: | A cDNA (Liv-RPCH) encoding a putative red pigment-concentrating hormone was cloned from whiteleg shrimp (Litopenaeus vannamei) eyestalks. The deduced amino acid sequence of this cDNA was 93 residues long and consisted of a 21-residue signal peptide, a 9-residue red pigment-concentrating hormone (RPCH) mature peptide, a dibasic cleavage site, and a 61-residue RPCH precursor-related peptide (RPRP). The Liv-RPCH transcript was detected not only in neural tissues (eyestalks, brain, thoracic ganglia, and abdominal ganglia) but also in non-neural tissues (ovaries and heart). In vivo injection of synthetic RPCH (pELNFSPGW-NH2) caused significant increases in hemolymph vitellogenin (Vg) concentrations, ovarian Vg mRNA levels, and mean oocyte area, suggesting that RPCH has a positive role in promoting ovarian growth in L. vannamei. The effects of 5-HT on the expression of Liv-RPCH transcripts in selected tissues (eyestalks, brain, thoracic ganglia, and ovaries) were examined by using quantitative real-time PCR. Injection of 5-HT (20 mu g g(-1) body weight) resulted in elevated Liv-RPCH transcript levels in the ovaries 3 and 6 h after injection, but no significant effects were observed in the eyestalks, brain, or thoracic ganglia. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20540 | ISSN: | 0044-8486 | DOI: | 10.1016/j.aquaculture.2018.04.027 |
Appears in Collections: | 06 CLEAN WATER & SANITATION |
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