http://scholars.ntou.edu.tw/handle/123456789/20591
標題: | Using metabolic charge production in the tricarboxylic acid cycle (Q(TCA)) to evaluate the extracellular-electron-transfer performances of Shewanella spp | 作者: | Li, Shiue-Lin Yen, Jui-Hung Kano, Kenji Liu, Shiu-Mei Liu, Chien-Liang Cheng, Sheng-Shung Chen, Hsun-Yi |
關鍵字: | MICROBIAL FUEL-CELLS;OUTER-MEMBRANE CYTOCHROMES;BACTERIAL NANOWIRES;MR-1;REDUCTION;TRANSPORT;RESPIRATION;MODEL;GEOBACTER;FLAVINS | 公開日期: | 十二月-2018 | 出版社: | ELSEVIER SCIENCE SA | 卷: | 124 | 起(迄)頁: | 119-126 | 來源出版物: | BIOELECTROCHEMISTRY | 摘要: | Using an electrochemical cell equipped with carbon felt electrodes (poised at +0.63 V vs. SHE), the current production capabilities of two Shewanella strains-NTOU1 and KR-12-were examined under various conditions with lactate as an electron donor. The metabolic charge produced in the tricarboxylic acid cycle (Q(TCA)) was calculated by mass-balance. The data showed a linear relation between the electric coulomb production (Q(EL)) and Q(TCA) with an R-2 of 0.65. In addition, a large amount of pyruvate accumulation was observed at pH = 6, rendering Q(TCA) negative. The results indicate an occurrence of an undesired cataplerotic reaction. It was also found that Q(TCA) provides important information showing the oxygen-boosting TCA cycle and anodic-current generation of Shewanella spp. Linear dependence of the change in charge for biomass growth (4.52F Delta n(cell)) on Q(TCA) was also found as expressed by 4.52F Delta n(cell) = 1.0428 Q(TCA) + 0.0442, indicating that these two charge quantities are inherently identical under most of the experimental conditions. In the mediator-spiked experiments, the external addition of the mediators (ferricyanide, anthraquinone-2, 6-disulfonate, and riboflavin) beyond certain concentrations inhibited the activity of the TCA cycle, indicating that the oxidative phosphorylation is deactivated by excessive amounts of mediators, yet Shewanella spp. are constrained with regard to carrying out the substrate level phosphorylation. (C) 2018 Elsevier B.V. All rights reserved. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20591 | ISSN: | 1567-5394 | DOI: | 10.1016/j.bioelechem.2018.07.001 |
顯示於: | 海洋生物研究所 07 AFFORDABLE & CLEAN ENERGY |
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