|Title:||Enhanced anti-inflammatory activity of brown seaweed Laminaria japonica by fermentation using Bacillus subtilis||Authors:||Lin, Hong-Ting Victor
Lu, Wen Jung
|Keywords:||NF-KAPPA-B;PROTEASE PRODUCTION;SULFATED POLYSACCHARIDE;BIOLOGICAL-PROPERTIES;ANTIOXIDANT ACTIVITY;PHENOLIC-COMPOUNDS;ETHANOLIC EXTRACT;OXIDATIVE STRESS;MARINE-ALGAE;HIGH-LEVEL||Issue Date:||Dec-2016||Publisher:||ELSEVIER SCI LTD||Journal Volume:||51||Journal Issue:||12||Start page/Pages:||1945-1953||Source:||PROCESS BIOCHEM||Abstract:||
Most studies on the anti-inflammatory effects of Laminaria japonica have investigated bioactive ingredients that have been extracted from Laminaria using organic solvents. However, to avoid the toxicity and environmental issues caused by the solvent used for extraction, increasing attention has focused on fermentation using microorganisms as a green bioconversion process for producing bioactive compounds. In this study, fermentation with Bacillus subtilis, where the cultivation parameters and nutrient additives were optimized to achieve the greatest inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production, was found to enhance the anti-inflammatory effects of L. japonica. Fermentation by B. subtilis using a 5% (w/v) L japonica suspension as the sole carbon source, 2% additional nitrogen source, a starting pH of 6, and incubation at 150 rpm at 37 degrees C for 72 h yielded the best anti-inflammatory effects, as LPS-induced NO production decreased to 27.6% +/- 5.2%. The macrophages treated with the fermented L japonica product inhibited the LPS-induced production of reactive oxygen species (ROS) and NF-kappa B (p65) phosphorylation in a concentration-dependent manner. Thus, our results show that L japonica products generated by an optimized fermentation process designed to enhance anti-inflammatory effects by decreasing ROS production, NF-kappa B (p65) phosphorylation, prostaglandin E-2, and NO production in macrophages. (C) 2016 Elsevier Ltd. All rights reserved.
|Appears in Collections:||生命科學暨生物科技學系|
14 LIFE BELOW WATER
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