http://scholars.ntou.edu.tw/handle/123456789/21468
Title: | Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Doxorubicin Nanoparticles of mPEG-PCL-Graft-Cellulose | Authors: | Chen, Chao-Hsuan Cuong, Nguyen-Van Yung-Tsung Chen So, Regina Cheng Liau, Ian Hsieh, Ming-Fa |
Keywords: | HUMAN BREAST CANCER CELLS;METHOXY-POLY(ETHYLENE GLYCOL);MULTIDRUG RESISTANCE;P-GLYCOPROTEIN;POLY(EPSI-CAPROLACTONE) | Issue Date: | Jan-2011 | Publisher: | AMER SCIENTIFIC PUBLISHERS | Journal Volume: | 11 | Journal Issue: | 1 | Start page/Pages: | 53-60 | Source: | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | Abstract: | The amphiphilic block copolymer methoxy-poly(ethylene glycol)-poly(ε-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce nano-sized micellar nanoparticles. The nanoparticles were loaded with anti-tumor drug, doxorubicin (DOX) and the size of the DOX-loaded nanoparticles were determined by dynamic light scattering (DLS) in aqueous solution to be from 197.4 to 230 nm. The nanoparticles subjected to co-culture with macrophage cells showed that these nanoparticles used as drug carrier are not recognized as foreign bodies. Overexpression of P-glycoprotein (P-gp) is an important factor in the development of multidrug resistance (MDR) in many cancer cells. In this study, Western blot and Rhodamine 123 were used to monitor the relative P-glycoprotein expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. The endocytosis of the DOX-loaded nanoparticles by breast cancer cells is more efficient observed under a confocal laser scanning microscopy (CLSM) and a flow cytometry in MCF7/ADR cells, compared to the diffusion of the free drug into the cytoplasm of cells. Based on these findings, we concluded that the nanoparticles made from mPEG-PCL-g-cellulose were effective in overcoming P-gp efflux in MDR breast cancer cells. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/21468 | ISSN: | 1533-4880 | DOI: | 10.1166/jnn.2011.3102 |
Appears in Collections: | 食品科學系 |
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