http://scholars.ntou.edu.tw/handle/123456789/21468
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen, Chao-Hsuan | en_US |
dc.contributor.author | Cuong, Nguyen-Van | en_US |
dc.contributor.author | Yung-Tsung Chen | en_US |
dc.contributor.author | So, Regina Cheng | en_US |
dc.contributor.author | Liau, Ian | en_US |
dc.contributor.author | Hsieh, Ming-Fa | en_US |
dc.date.accessioned | 2022-04-26T02:22:33Z | - |
dc.date.available | 2022-04-26T02:22:33Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/21468 | - |
dc.description.abstract | The amphiphilic block copolymer methoxy-poly(ethylene glycol)-poly(ε-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce nano-sized micellar nanoparticles. The nanoparticles were loaded with anti-tumor drug, doxorubicin (DOX) and the size of the DOX-loaded nanoparticles were determined by dynamic light scattering (DLS) in aqueous solution to be from 197.4 to 230 nm. The nanoparticles subjected to co-culture with macrophage cells showed that these nanoparticles used as drug carrier are not recognized as foreign bodies. Overexpression of P-glycoprotein (P-gp) is an important factor in the development of multidrug resistance (MDR) in many cancer cells. In this study, Western blot and Rhodamine 123 were used to monitor the relative P-glycoprotein expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. The endocytosis of the DOX-loaded nanoparticles by breast cancer cells is more efficient observed under a confocal laser scanning microscopy (CLSM) and a flow cytometry in MCF7/ADR cells, compared to the diffusion of the free drug into the cytoplasm of cells. Based on these findings, we concluded that the nanoparticles made from mPEG-PCL-g-cellulose were effective in overcoming P-gp efflux in MDR breast cancer cells. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | en_US |
dc.relation.ispartof | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | en_US |
dc.subject | HUMAN BREAST CANCER CELLS | en_US |
dc.subject | METHOXY-POLY(ETHYLENE GLYCOL) | en_US |
dc.subject | MULTIDRUG RESISTANCE | en_US |
dc.subject | P-GLYCOPROTEIN | en_US |
dc.subject | POLY(EPSI-CAPROLACTONE) | en_US |
dc.title | Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Doxorubicin Nanoparticles of mPEG-PCL-Graft-Cellulose | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1166/jnn.2011.3102 | - |
dc.identifier.isi | WOS:000286344400005 | - |
dc.relation.journalvolume | 11 | en_US |
dc.relation.journalissue | 1 | en_US |
dc.relation.pages | 53-60 | en_US |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.orcid | https://orcid.org/0000-0002-6714-0548 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 食品科學系 |
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