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  1. National Taiwan Ocean University Research Hub
  2. 生命科學院
  3. 食品安全與風險管理研究所
Please use this identifier to cite or link to this item: http://scholars.ntou.edu.tw/handle/123456789/22054
Title: In Silico and In Vitro Analyses of Angiotensin-I Converting Enzyme Inhibitory and Antioxidant Activities of Enzymatic Protein Hydrolysates from Taiwan Mackerel (Scomber australasicus) Steaming Juice
Authors: Panjaitan, Fenny Crista A.
Chen, Ting-Yi
Ku, Hao-Hsiang 
Chang, Yu-Wei 
Keywords: TUNA COOKING JUICE;PEPTIDES;PURIFICATION;PRESSURE
Issue Date: Jun-2022
Publisher: MDPI
Journal Volume: 11
Journal Issue: 12
Source: FOODS
Abstract: 
Mackerel (Scomber australasicus) steaming juice (MSJ) can be a good source of proteins. However, it is often treated as food waste during the canning process. The objective of this study was to investigate the Angiotensin-I converting enzyme (ACE-I) inhibitory and antioxidant activities from MSJ hydrolysates using in silico and in vitro approaches. Proteins extracted from MSJ were identified by proteomic techniques, followed by sulfate polyacrylamide gel electrophoresis (SDS-PAGE), in-gel digestion, tandem mass spectrometry and on-line Mascot database analysis. Myosin heavy chain (fast skeletal muscle), actin, myosin light chain 1 (skeletal muscle isoform), collagen alpha-2(I) chain, tropomyosin alpha-1 chain, beta-enolase, fructose-bisphosphate aldolase A and glyceraldehyde-3- phosphate dehydrogenase were identified and further analyzed using BIOPEP-UWM database. In silico results indicated that MSJ proteins had potential bioactive peptides of antioxidant and ACE-I inhibitory activities. MSJ was then hydrolyzed using six proteases (papain, pepsin, proteinase k, alcalase, bromelain, thermolysin). In particular, pepsin hydrolysates (5 mg/mL) showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (61.54%) among others. Alcalase hydrolysates (5 mg/mL) exhibited the highest metal chelating activity (89.76%) and proteinase K hydrolysates (5 mg/mL) indicated the highest reducing power activity (1.52 abs). Moreover, pepsin hydrolysates (0.1 mg/mL) possessed the highest ACE inhibitory activity (86.15%). Current findings suggest that MSJ hydrolysates can be a potential material to produce ACE-I inhibitory and antioxidant peptides as nutraceutical or pharmaceutical ingredients/products with added values.
URI: http://scholars.ntou.edu.tw/handle/123456789/22054
ISSN: 2304-8158
DOI: 10.3390/foods11121785
Appears in Collections:食品安全與風險管理研究所
食品科學系
11 SUSTAINABLE CITIES & COMMUNITIES
12 RESPONSIBLE CONSUMPTION & PRODUCTION

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