|Title:||Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein||Authors:||Chen, Han-Hsiang
Tseng, Yufeng Jane
Wang, Robert Y. L.
|Keywords:||DENGUE VIRUS;GRAPHENE OXIDE;FULLERENE DERIVATIVES;CRYSTAL-STRUCTURE;CURCUMIN;INHIBITION;ENTRY;NS3;NANOMATERIALS;MUTATION||Issue Date:||Jun-2022||Publisher:||ELSEVIER||Journal Volume:||298||Journal Issue:||6||Source:||J BIOL CHEM||Abstract:||
Japanese encephalitis is a mosquito-borne disease caused by the Japanese encephalitis virus (JEV) that is prevalent in Asia and the Western Pacific. Currently, there is no effective treatment for Japanese encephalitis. Curcumin (Cur) is a compound extracted from the roots of Curcuma longa, and many studies have reported its antiviral and anti-inflammatory activities. However, the high cytotoxicity and very low solubility of Cur limit its biomedical applications. In this study, Cur carbon quantum dots (Cur-CQDs) were synthesized by mild pyrolysis-induced polymerization and carbonization, leading to higher water solubility and lower cytotoxicity, as well as superior antiviral activity against JEV infection. We found that CurCQDs effectively bound to the E protein of JEV, preventing viral entry into the host cells. In addition, after continued treatment of JEV with Cur-CQDs, a mutant strain of JEV was evolved that did not support binding of Cur-CQDs to the JEV envelope. Using transmission electron microscopy, biolayer interferometry, and molecular docking analysis, we revealed that the S123R and K312R mutations in the E protein play a key role in binding Cur-CQDs. The S123 and K312 residues are located in structural domains II and III of the E protein, respectively, and are responsible for binding to receptors on and fusing with the cell membrane. Taken together, our results suggest that the E protein of flaviviruses represents a potential target for the development of CQD-based inhibitors to prevent or treat viral infections.
|Appears in Collections:||生命科學暨生物科技學系|
03 GOOD HEALTH AND WELL-BEING
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.