|Title:||A New Biorecognition-Element-Free ID mu E Sensor for the Identification and Quantification of E. coli||Authors:||Lin, Yung-Kai
Nguyen Van Hieu
Thi Vien Thao Do
Yao, Fiona Yan-Dong
Thien Luan Phan
Ching, Congo Tak Shing
|Keywords:||Escherichia coli;interdigitated microelectrodes;biorecognition element free;impedance;capacitance||Issue Date:||1-Aug-2022||Publisher:||MDPI||Journal Volume:||12||Journal Issue:||8||Source:||BIOSENSORS-BASEL||Abstract:||
The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as Escherichia coli as a preventive measure. In this study, a biorecognitionelement-free interdigitated microelectrode (ID mu E) sensor is designed and developed with this in mind, with good reliability and affordability. Results show that the designed sensor can identify E. coli with good selectivity using an impedance and capacitance of 7.69 MHz. At its optimum impedance of 1.3 kHz, the ID mu E sensor can reliably quantify E. coli in a range of measurement (10(3.2) similar to 10(6) cfu/mL), linearity (R-2 = 0.97), sensitivity (18.15 k Omega/log (cfu/mL)), and limit of detection (10(3.2) cfu/mL). In summary, the ID mu E sensor developed possesses high potential for industrial and clinical applications.
|Appears in Collections:||食品安全與風險管理研究所|
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