http://scholars.ntou.edu.tw/handle/123456789/3571
標題: | EGR1 upregulation following Venezuelan equine encephalitis virus infection is regulated by ERK and PERK pathways contributing to cell death | 作者: | Bibha Dahal Shih-Chao Lin Brian D. Carey Jonathan L. Jacobs Jonathan D. Dinman Monique L. van Hoek Andre A. Adams Kylene Kehn-Hall |
關鍵字: | EGR1;Early growth response 1;Venezuelan equine encephalitis virus;VEEV;Apoptosis;ERK;PERK;Unfolded protein response;UPR;Alphavirus | 公開日期: | 2-一月-2020 | 出版社: | Elsevier | 卷: | 539 | 起(迄)頁: | 121-128 | 來源出版物: | Virology | 摘要: | Venezuelan equine encephalitis virus (VEEV) is a neurotropic virus that causes significant disease in both humans and equines. Here we characterized the impact of VEEV on signaling pathways regulating cell death in human primary astrocytes. VEEV productively infected primary astrocytes and caused an upregulation of early growth response 1 (EGR1) gene expression at 9 and 18 h post infection. EGR1 induction was dependent on extracellular signal-regulated kinase1/2 (ERK1/2) and protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), but not on p38 mitogen activated protein kinase (MAPK) or phosphoinositide 3-kinase (PI3K) signaling. Knockdown of EGR1 significantly reduced VEEV-induced apoptosis and impacted viral replication. Knockdown of ERK1/2 or PERK significantly reduced EGR1 gene expression, dramatically reduced viral replication, and increased cell survival as well as rescued cells from VEEV-induced apoptosis. These data indicate that EGR1 activation and subsequent cell death are regulated through ERK and PERK pathways in VEEV infected primary astrocytes. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/3571 | ISSN: | 0042-6822 | DOI: | 10.1016/j.virol.2019.10.016 |
顯示於: | 海洋生物科技學士學位學程(系) |
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