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Please use this identifier to cite or link to this item: http://scholars.ntou.edu.tw/handle/123456789/3626
Title: Identification and Characterization of an Extracellular Alkaline Phosphatase in the Marine Diatom Phaeodactylum tricornutum
Authors: Hung-Yun Lin 
Chi-Yu Shih
Hung-Chun Liu
Jeng Chang 
Ying-Lan Chen
Yet-Ran Chen
Han-Tso Lin
Yu-Yung Chang
Chun-Hua Hsu
Han-Jia Lin 
Keywords: EMILIANIA-HUXLEYI PRYMNESIOPHYCEAE;TRANSCRIPTIONAL REGULATION;GENE-EXPRESSION;SEQUENCE;LIMITATION;STRESS;MECHANISM;EVOLUTION;MATRIX;WATERS
Issue Date: Aug-2013
Publisher: SPRINGER
Journal Volume: 15
Journal Issue: 4
Start page/Pages: 425-436
Source: Marine Biotechnology
Abstract: 
In phosphorus-deficient conditions, Phaeodactylum tricornutum releases an alkaline phosphatase (PtAPase) to the medium that is readily detectable by activity staining. Nucleic acid and amino acid sequence of this alkaline phosphatase (APase) was identified by performing proteomic analysis and database searches. Sequence alignment suggests that PtAPase belongs to the PhoA family, and it possesses key residues at the Escherichia coli PhoA active site. Quantitative PCR results indicate that the induction of APase mRNA transcription is very sensitive to phosphorus availability and population growth. The molecular mass of native PtAPase (148 kDa) determined by gel filtration chromatography indicates that PtAPase, like most PhoA, is homodimeric. Zn and Mg ions are essential cofactors for most PhoA enzymes; however, PtAPase activity did not require Zn ions. In fact, 5 mM Zn2+, Mo2+, Co2+, Cd2+, or Cu2+ inhibited its enzymatic activity, whereas 5 mM Mn2+, Mg2+, or Ca2+ enhanced its enzymatic activity. The responses of PtAPase to divalent metal ions were different from those of most PhoAs, but were similar to the PhoA in a marine bacterium, Cobetia marina. Phylogenetic analysis shows that homologs of PhoA are also present in other diatom species, and that they clustered in a unique branch away from other PhoA members. PtAPase may represent a novel class of PhoA that helps diatoms to survive in the ocean. Quantification of the PtAPase mRNA may help monitor the physiological condition of diatoms in natural environments and artificial bioreactors.
URI: http://scholars.ntou.edu.tw/handle/123456789/3626
ISSN: 1436-2228
DOI: 10.1007/s10126-013-9494-3
Appears in Collections:海洋生物研究所

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