|Title:||Study on Chemical Profile and Neuroprotective Activity of Myrica rubra Leaf Extract||Authors:||Chen, Pinghong
|Keywords:||FLIGHT MASS-SPECTROMETRY;OXIDATIVE STRESS;DIARYLHEPTANOID GLYCOSIDES;PHENOLIC-COMPOUNDS;CALCIUM OVERLOAD;HPLC-DAD;CONSTITUENTS;IDENTIFICATION;CELLS;ANTIOXIDANT||Issue Date:||Jul-2017||Publisher:||MDPI||Journal Volume:||22||Journal Issue:||7||Source:||MOLECULES||Abstract:||
The chemical profile of Myrica rubra (a native species in China) leaf extract was investigated by UPLC-PDA-HRMS, and the neuroprotective activity of two characteristic constituents, myricanol and myricetrin, was evaluated with N2a cells using H2O2-inducedoxidative challenge through a series of methods, e.g., MTT assay, ROS assay and [ Ca2+] i assay. Among the 188 constituents detected in the extract of Myrica rubra leaf, 116 were identified definitely or tentatively by the comprehensive utilization of precise molecular weight and abundant multistage fragmentation information obtained by quadrupole orbitrap mass spectrometry. In addition, 14 potential new compounds were reported for the first time. This work established an example for the research of microconstituents in a complex analyte and revealed that suppression of H2O2-induced cytotoxicity in N2a cells was achieved by the pretreatment with myricanol. The evidence suggested myricanol may potentially serve as a remedy for prevention and therapy of neurodegenerative diseases induced by oxidative stress.
|Appears in Collections:||食品安全與風險管理研究所|
15 LIFE ON LAND
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.