http://scholars.ntou.edu.tw/handle/123456789/5896
Title: | Oligo-Fucoidan Prevents M2 Macrophage Differentiation and HCT116 Tumor Progression | Authors: | Chen, Li-Mei Tseng, Hong-Yu Chen, Yen-An Al Haq, Aushia Tanzih Hwang, Pai-An Hsu, Hsin-Ling |
Keywords: | OXIDATIVE STRESS;MESENCHYMAL TRANSITION;SUPEROXIDE-DISMUTASE;DRUG-RESISTANCE;IN-VITRO;CANCER;ROS;DEGRADATION;METASTASIS;ACTIVATION | Issue Date: | Feb-2020 | Publisher: | MDPI | Journal Volume: | 12 | Journal Issue: | 2 | Source: | CANCERS | Abstract: | Reactive oxygen species (ROS) produced during intracellular metabolism or triggered by extrinsic factors can promote neoplastic transformation and malignant microenvironment that mediate tumor development. Oligo-Fucoidan is a sulfated polysaccharide isolated from the brown seaweed. Using human THP-1 monocytes and murine Raw264.7 macrophages as well as human HCT116 colorectal cancer cells, primary C6P2-L1 colorectal cancer cells and human MDA-MB231 breast cancer cells, we investigated the effect of Oligo-Fucoidan on inhibiting M2 macrophage differentiation and its therapeutic potential as a supplement in chemotherapy and tumor prevention. We now demonstrate that Oligo-Fucoidan is an antioxidant that suppresses intracellular ROS and mitochondrial superoxide levels in monocytes/macrophages and in aggressive cancer cells. Comparable to ROS inhibitors (DPI and NAC), Oligo-Fucoidan directly induced monocyte polarization toward M1-like macrophages and repolarized M2 macrophages into M1 phenotypes. DPI and Oligo-Fucoidan also cooperatively prevented M2 macrophage invasiveness. Indirectly, M1 polarity was advanced particularly when DPI suppressed ROS generation and supplemented with Oligo-Fucoidan in the cancer cells. Moreover, cisplatin chemoagent polarized monocytes and M0 macrophages toward M2-like phenotypes and Oligo-Fucoidan supplementation reduced these side effects. Furthermore, Oligo-Fucoidan promoted cytotoxicity of cisplatin and antagonized cisplatin effect on cancer cells to prevent M2 macrophage differentiation. More importantly, Oligo-Fucoidan inhibited tumor progression and M2 macrophage infiltration in tumor microenvironment, thus increasing of anti-tumor immunity. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/5896 | ISSN: | 2072-6694 | DOI: | 10.3390/cancers12020421 |
Appears in Collections: | 生命科學暨生物科技學系 03 GOOD HEALTH AND WELL-BEING |
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