http://scholars.ntou.edu.tw/handle/123456789/6159
標題: | Omega-3 polyunsaturated fatty acids suppress metastatic features of human cholangiocarcinoma cells by suppressing twist | 作者: | Chia-Ray Lin Tsan-Mei Chu Anin Luo Shin-Jie Huang Hsin-Yiu Chou Ming-Wei Lu Jen-Leih Wu |
關鍵字: | INTRAHEPATIC CHOLANGIOCARCINOMA;DOCOSAHEXAENOIC ACID;COLORECTAL-CANCER;PERINEURAL INVASION;DOWN-REGULATION;BETA-CATENIN;OMEGA-3-FATTY-ACIDS;TRENDS;MECHANISMS;PREVENTION | 公開日期: | 十二月-2019 | 出版社: | ELSEVIER | 卷: | 74 | 來源出版物: | The Journal of Nutritional Biochemistry | 摘要: | Cholangiocarcinoma (CCA) is a highly malignant cancer of the bile duct, which has a five-year survival rate less than 5% due to a high metastasis rate and lack of therapeutic options. Although omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been shown to inhibit the proliferation of CCA cells, the effects on CCA metastasis have not been previously reported. In this study, we first assessed the proliferation, migration and invasion effects of n-3 PUFA-based fish oil on human CCA cells. Then, we investigated PUFA effects on metastasis in vivo by xenografting CCA cells into zebrafish larvae that overexpress a critical n-3 PUFA synthesis gene, Delta 6 fatty acid desaturase. The results indicated that n-3 PUFA-based fish oil suppresses CCA cell growth, potentially by blocking the cell cycle at G(2)/M phase, and it inhibits migration and invasion potential with coincident downregulation of migration-related genes. Furthermore, zebrafish endogenous n-3 PUFAs appear to suppress CCA metastasis by inhibiting the expression of twist, a key regulator of tumor metastasis. Interestingly, only long chain n-3 PUFAs could inhibit the expression of twist in CCA cells. Together, our results suggest that n-3 PUFAs, especially DHA, may inhibit proliferation and metastasis of CCA cells by inhibiting the expression of twist. (C) 2019 Elsevier Inc. All rights reserved. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/6159 | ISSN: | 0955-2863 | DOI: | 10.1016/j.jnutbio.2019.108245 |
顯示於: | 水產養殖學系 03 GOOD HEALTH AND WELL-BEING |
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