The Regulation of Interferon Signaling in Nervous Necrosis Virus Infection

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簡歷

基本資料

Project title

Code/計畫編號

NSC102-2313-B019-011

Translated Name/計畫中文名

神經壞死病毒對於干擾素訊息傳遞的調控

Project Coordinator/計畫主持人

Ming-Wei Lu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=3095017

Year

2013

Start date/計畫起

01-08-2013

Expected Completion/計畫迄

31-07-2014

Bugetid/研究經費

820千元

ResearchField/研究領域

漁業

"Interferon 對魚類的先天性免疫來說扮演著極為重要的角色，尤其在抗病毒方面的研究更是不勝枚舉。斑馬魚 type I interferon (zfIFNI)根據他們的蛋白質序列可歸類成 group I (zfIFNØ1 和 zfIFNØ4) 及 group II (zfIFNØ2 和 zfIFNØ3)，而我們的分析健康與感染神經壞死病毒之斑馬魚後，發現其 type I interferon 的表現模式各不相同。有鑑於以上幾點，本計劃欲了解不同的 type I interferon 所調控的免疫基因，利用斑馬魚生長、分化快速及全基因體定序已完成之優勢，整理出各個 interferon 對於在抗病毒上所扮演的角色、所調控的下游基因以及基因的表現差異，使整個 interferon 路徑更佳的明確，完成斑馬魚上的 interferon 下游基因訊息傳遞路徑後，我們也將會將其驗證在石斑魚上。關於石斑魚 interferon 的資訊目前較少，目前我們已經完成石斑魚轉錄體的定序，利用這個資料庫再加上本計畫第一年斑馬魚實驗所得的成果，將可以了解以下的重點項目:1.魚類第一型干擾素的作用機制及功能，2.第一型干擾素在神經壞死病毒感染上所扮演的角色。" "Interferons (IFNs) are cytokines involved in fish innate immune responses, especially in against viral pathogens. Zebrafish type I interferon (zfIFNI) can be categorized into group I (zfIFNØ1 and zfIFNØ4) and group II (zfIFNØ2 and zfIFNØ3) according to their primary protein sequences. In our previous study, we found that the differential expressions of type I IFNs healthy and nervous necrosis virus (NNV) infected zebrafish. From the result above mentioned, we wish to examine the regulations of immune genes by different type I IFNs. With the advantages of fast growth, fast differentiation properties of zebrafish and completion of its whole genome sequencing, we wish to have a clearer overview of IFN pathway, by examine the roles of IFNs in antiviral activity, and the regulation and expression of downstream genes. This information of zfIFN can be applied as a reference in grouper IFN pathway, which will be useful for the antiviral research in grouper."

DSpace CRIS

The Regulation of Interferon Signaling in Nervous Necrosis Virus Infection

基本資料

Description