Tissue Patterning Polymorphism via Signaling Dynamics in Notch Signaling Pathway(II)

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基本資料

Project title

Code/計畫編號

MOST108-2311-B019-001-MY3

Translated Name/計畫中文名

Notch訊息傳遞動態所調控之組織圖樣化多型性(II)

Project Coordinator/計畫主持人

Bo-Kai Liao

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

周銘翊

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=13334056

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

1470千元

ResearchField/研究領域

生物技術(理)

"複雜的身體結構由圖樣化的胚胎組織中出現，而這些自組成細胞圖樣本身是如何產生的，則是生物學中的一個基礎問題，但卻涉及演化過程中身體結構之轉變和遺傳性疾病的發展等。儘管組織圖樣化之機制仍不完全清楚，但其通常是透過相鄰細胞之間的訊息傳遞來形成，其一常見的系統便是Delta-Notch的訊息傳遞。有趣的是，使用相同的主要訊息傳遞元件之不同組織常出現兩種概念相反的圖樣，例如細胞相互抑制之側邊抑制與相互促進之細胞振盪器同步化，這暗示著訊息傳遞動力學與訊息時間才是決定不同圖樣化之關鍵因素，而非該細胞組織本身。這樣的過程被稱為組織圖樣化多樣性，然而，涉及此圖樣化多樣性之關鍵機制仍尚未被解開，不同的組織圖樣仍以不同訊息傳遞數學模式進行解釋。在此，本研究提出了包括全胚胎活體影像、合成生物學和系統生物學之跨領域策略，用以解決組織圖樣化多樣性的四個基本挑戰：（1）在Notch訊息傳遞過程中細胞命運決定機制是如何達成的;（2）於不同組織圖樣間，其Notch訊息傳遞之穩健性的差異為何; （3）改寫細胞指令以變換組織圖樣所需之關鍵因子為何; （4）透過數學模式建立統一理論來全面描述組織圖樣化多樣性。本研究計劃旨在為Notch訊息傳遞動態提供更高層次的理解，並為潛在的應用提供紮實的基礎。" "Complex body structures emerge from patterned embryonic tissues. How these embryonic patterns are themselves generated is a fundamental question of biology, with implications for the transformation of body structure throughout evolution and the development of inherited disease. Although details of how embryonic tissue patterns are not completely clear, they are often shaped by exchange of signals between neighboring cells, largely via a signaling system called Delta-Notch. Intriguingly, the same major biochemical components are often found in two conceptually opposite scenarios, lateral inhibition and oscillator synchronization for example, suggesting that dynamics and timing are the key factors determining the different patterning outcomes. This is a situation termed patterning polymorphism; however, how the signaling dynamics involving in this process is still unclear. Here, multidisciplinary strategies, including in toto live imaging, synthetic biology and systems biology, are proposed to address four fundamental challenges of patterning polymorphism: (1) how the cell fate determination is defined during Notch signaling; (2) what are the robustness difference in different Notch signaling patterned tissues; (3) what are the key factors required for rewriting cell instructions to cause altered tissue patterns; and (4) how to establish a unification theory by mathematical modeling to describe tissue patterning polymorphism. This research plan is aimed to provide the next level of understanding to Notch signaling dynamics, and to support a solid foundation for potential applications."

Keyword(s)

訊息傳遞動態
系統生物學
合成生物學
選擇平面照明顯微鏡
數學模式
組織圖像化多樣性
Notch signaling dynamics
Systems biology
Synthetic biology
Selective plane illumination microscopy
mathematical modeling
Tissue patterning polymorphism

DSpace CRIS

Tissue Patterning Polymorphism via Signaling Dynamics in Notch Signaling Pathway(II)

基本資料

Description