Characterization of Grouper Iridovirus on the Continuous Cells from Grouper (Epinephelus sp.)

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基本資料

Project title

Code/計畫編號

90農科-1.4.5-漁-F3(16)

Translated Name/計畫中文名

石斑虹彩病毒在石斑魚株化細胞之特性研究

Project Coordinator/計畫主持人

Hsin-Yiu Chou

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1046375

Year

2001

Start date/計畫起

01-01-2001

Expected Completion/計畫迄

31-12-2001

Bugetid/研究經費

720千元

ResearchField/研究領域

漁業
生物科學

計畫目標: 1995年本省養殖石斑魚爆發疑似病毒感染所造成之魚苗的大量死亡, 以穿透式電子顯微鏡觀察罹病魚, 結果發現許多成群的六角形病毒粒子, 進而分離出病毒.根據此病毒一般基本特性及病毒的大小與形態, 推測其屬於虹彩病毒科( Iridoviridae ), 暫時將其命名為石斑虹彩病毒( grouper iridovirus of Taiwan ), 簡稱TGIV.而此病毒之人工感染實驗結果顯示, TGIV對石斑魚苗具有高病原性會引發高死亡率, 此外, 黃錫鯛和黃鰭鯛對TGIV也有高感受性.此病毒的高毒性與廣泛的寄主範圍, 對本省海水養殖業有潛在威脅.曾嘗試以多種魚類細胞株進行相關研究, 但這些細胞感受性低且穩定性差, 隨繼代次數的增加, 力價明顯下降, 造成研究上之困難.為了有效增殖TGIV, 本計劃擬探討TGIV在由石斑魚株化細胞上之增殖情形、繼代之穩定性以及感染力與病原性之變化, 希望能建立TGIV之有效培養系統, 將來有效地應用於病毒純化與疫苗開發之相關研究.重要工作項目: ( 一 )TGIV在不同細胞增殖情形之觀察.( 二 )繼代之穩定性.( 三 )保存溫度之測定.( 四 )病毒之純化.預期效益: 藉由TGIV在不同細胞增殖情形以及繼代穩定性之探討, 除可純化病毒進而和其他虹彩病毒進行比較外, 將來更能有效地應用於疫苗開發相關之防治研究. An epizootic viral disease has threatened cultured grouper ( Epinephelus sp. )in Taiwan since 1995.Icosahedral viral particles were observed under electron microscopy in the moribund fish.A viral agent was isolated from diseased grouper.Based on its general properties and viral morphology, we propose the name Grouper Iridovirus of Taiwan ( TGIV ).The result of pathogenicity test demonstrates that TGIV was highly pathogenic to grouper.Besides, silver bream ( Sparus sarba )and black sea bream ( Acanthopagrus latus )are both susceptible to infection with TGIV.Due to its virulence and broad host range, the potential menace of this iridoviral agent to marine aquaculture industry is undoubtedly.As mentioned in our previously study, TGIV quantity and quality declined rapidly during serial passages of KRE cells.This instability of TGIV in cells is a barrier to develop control measures for the virus.In this project, attempts were made to perform a more effective culture system and to increase virus yield.We believe that such a culture system will potentially be of value in further study of the control of the diseases.The project included as follows: ( 1 )Multiplication of TGIV on different cells.( 2 )Stability of TGIV during serial passages.( 3 )Stability of TGIV under different storage temperatures.( 4 )TGIV purification.A better culture of TGIV will help to establish information for vaccine development and control of the disease, as well as determine the DNA structure and other specific characteristics of this virus.

DSpace CRIS

Characterization of Grouper Iridovirus on the Continuous Cells from Grouper (Epinephelus sp.)

基本資料

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