Development and Application of Fish Iridoviral Vaccine---Effectiveness and Safeness Evaluation in Field Trials

View Statistics Email Alert RSS Feed

Information

Details

Project title

Code/計畫編號

95農科-6.1.6-漁-F1(2)

Translated Name/計畫中文名

魚類虹彩病毒疫苗之研發與應用

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Co-Investigator(s)/共同執行人

張繼堯

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1270187

Year

2006

Start date/計畫起

01-04-2006

Expected Completion/計畫迄

31-12-2006

Bugetid/研究經費

1400千元

ResearchField/研究領域

漁業

疫苗開發雖是解決魚類病毒性疾病的重要防治對策之一，但成本偏高、施用方式的選擇、以及緊迫與有效性等問題仍待解決，因此目前只有極少數的疫苗成為商品化。本計劃擬針對石斑虹彩病毒研發口服疫苗製劑和次單位疫苗。可是口服疫苗會被魚體的防禦機制與胃酸和腸胃道酵素分解，使得這些抗原物質還未到達目標器官即被破壞殆盡，無法被吸收誘發保護作用。因此本年度將嘗試以乳化處理改善疫苗經口免疫的問題，再進行田間試驗以評估口服疫苗的有效性和安全性。另外安全性高且可利用遺傳工程技術大量生產的次單位疫苗研發也將同時進行。將針對六個已完成定序、表現與純化的GIV重組蛋白質 (010R, 027L, 028L, 042L, 049L, 078R)，製備其抗體後進行體外病毒的中和試驗，最後根據產生中和抗體之能力，選出最適作為次單位疫苗之重組蛋白質。本年度希望能研發出有效且安全的虹彩病毒疫苗，同時進行田間試驗，確認虹彩病毒疫苗現場應用之可行性。 Vaccine development is one of the major approaches of controlling viral diseases in aquaculture. Unfortunately, in consideration of detailed items of vaccination, such as cost, stress and efficacy, there are only a few of commercially available vaccines for fish viral pathogens to date. In this project, aiming at practical uses, attempts were made to develop emulsified oral vaccine and subunit vaccine against grouper iridovirus simultaneously. Since gastric acid and gastrointestinal enzymes of fish damage antigencity of oral vaccine frequently. It is quite difficult to induce protection in the route of oral administration. In order to improve the efficacy of oral vaccine, emulsion conditions of oral vaccine preparation will be studied. Sequentially, effectiveness and safeness of this emulsified oral vaccine will be evaluated in field trials. Besides, six successfully expressed and purified GIV recombinant protein (010R, 027L, 028L, 042L, 049L, 078R) will be tested for subunit vaccine. The polyclonal antibody preparation is completed, and the neutralization efficacy against GIV is under proceeding. Finally, we hope to that present study will potentially be of value for practicable use and control of the iridoviral disease.

DSpace CRIS

Development and Application of Fish Iridoviral Vaccine---Effectiveness and Safeness Evaluation in Field Trials

Details

Description