Aquatic Antimicrobial Peptides in Cosmetics

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Project title

Code/計畫編號

NSC98-2324-B019-001

Translated Name/計畫中文名

應用水生動物抗菌胜太作為化妝品添加劑

Project Coordinator/計畫主持人

Jenn-Kan Lu

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

吳金洌

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1899987

Year

2009

Start date/計畫起

01-08-2009

Expected Completion/計畫迄

31-07-2010

Bugetid/研究經費

2000千元

ResearchField/研究領域

生物技術(農)
農業化學
漁業

"目前個人衛生用品（包括所有化妝品、保養品、洗髮精、牙膏等）都含有數種以上的防腐劑preservatives，它們常常是引起臉部紅、癢、刺痛。而化妝品常用防腐劑polymethybren 長期使用，甚至有可能導致致癌的危險。故尋找無毒、無副作用、生物可分解的天然防腐劑具有巨大商機。在過去抗菌肽的研究上發現，抗菌肽可以對革蘭氏陽性菌、革蘭氏陰性菌、真菌及原生動物都有破壞之能力。本計畫之長程目標為：一）以先前所研發出之水產生物抗菌肽作為殺死（抑制）微生物物質，達到無病原菌狀態，作為化妝品之天然防腐添加劑。為達成上述目標我們將先行進行各水產生物抗菌肽抗菌試驗（MIC、 MBC）、抗病毒）試驗。並且決定應用於化妝品與保養品中之最適添加濃度，並測試防腐效果。為了促進油脂與水分子之交互相融，乃有介面活性劑（surfactant）之應用。惟過去的介面活性劑都是化學介面活性劑，對環境生態常有毒害或其代謝衍生物有害。而由微生物生產的生物介面活性劑具有與化學合成介面活性劑同等之乳化作用，且低（或無）毒，可以為生物降解、消化，不會在環境中殘留，對環境生態友善。本計畫之研究目標二，為使用先前所分離出一種微生物抗菌肽(surfactin)其亦為脂胜肽產物，它不但具有抗菌、抗病毒能力，且具有清潔、乳化、皮膚穿透加強劑等特性，我們將之應用於個人清潔用品，化妝品、軟膏與其他藥品中。本計畫先將進行surfactin 突變株篩選、探討生產surfactin 最適發酵條件，及功能測定，包括油脂乳化試驗、起泡沫試驗並用老鼠進行皮膚穿透等試驗。初期將以老鼠進行皮膚穿透試驗。計畫後期將與臨床皮膚科醫師進行水產生物抗菌肽作為化妝品添加劑臨床試驗。" "At present personal hygiene products (include all cosmetics, skin care products, shampoo, toothpaste, etc.) containing several antiseptic preservatives, which might cause face red, itch, shouting pain often. The daily using cosmetic containing a common preservative such as polymethybren for a long term use, it may cause the carcinogenic effect. Therefore, the natural antimicrobial materials with nontoxic, no side-effect, biodegradable, environmental friendly characters have enormous business opportunity. Previous study have found that anitimicrobial peptides have bactericidal activities against such as Gram positive bacteria, Gram negative bacteria, fungi, protozoa, parasite and coated virus. The long term goal of present study is employment of aquatic AMP to suppress microbes, reaching has pathogens free states, as a natural preservative additive in the cosmetics and skin care products. After AMP added the cosmetic and skin care products will be tested the bacterocidal activities ( such as MIC, MBC and antivirus) will be performed to determine the optimum dosage. To promote the emulsification of the oil and water, surfactant is applied . In the past most surfactants are chemical surfactants, harmful to often have poisonning or its supersession derivative to the environmental ecology as well. Therefore, Natural surfactants produced from microorganisms, plants or animals are referred to as biosurfactants which are biodegradable, friendly to the environmental ecology is needed. The results of our previous studies demonstrated that this novel surfactin molecule has both anti-bacterial, anti-fungal and anti-virus effects. It suggests that surfactin might be has the potential as a medicine. Surfactin not only has bacteriocidal and anti-virus ability, but it also has emulsification, forming and increase surface tension. Another goal in present proposal is to using patented microbial AMP (also is a biosurfactant, surfactin) added into cosmetics and skin care products as an additives which will be served as a novel preservative, emulsifier, foamer, and transdermal penetration enhancer as well. In present study, the surfactin will mix with cosments and/or skin care products and skin cleanness, oil emulsification, and surface tension abilities will be analyzed. Since the AMP can produced temporary “pore”on cell surface, therefore, the ability of AMP or surfactin which served as transdermal penetration enhancers will be determined. The skin transdermal penentration enhancing test will be carried out to testing the penetration rate of different molelecules, such as fluorescent molecules, HA, collagen, g-PGA and mesomethodern by using a mouse skin model. At the end, we will collaborate which clinical dermatologist to carry out the clinical trial of these AMP additives in cosmetics, skin care products, ointment or other medicines."

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Aquatic Antimicrobial Peptides in Cosmetics

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