Development of Infertility Technology in Transgenic Fluorescent Ornamental Fish (II)

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Project title

Code/計畫編號

MOST104-2622-B019-001-CC1

Translated Name/計畫中文名

基因轉殖螢光觀賞魚之不孕技術開發( II )

Project Coordinator/計畫主持人

Hong-Yi Gong

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

胡紹揚
吳金洌

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=11483837

Year

2015

Start date/計畫起

01-06-2015

Expected Completion/計畫迄

31-05-2016

Bugetid/研究經費

1500千元

ResearchField/研究領域

生物技術(農)
漁業

"觀賞魚產業是精緻的養殖產業，為台灣農業生技發展重點項目之ㄧ。台灣在 2010 年6 月由芝林公司與中研院合作開發出全球首例之中型螢光轉基因神仙魚與九間波羅，因此在全球基因轉殖觀賞魚產業上具有技術領導地位。中型螢光神仙魚(Pterophyllum scalare) 與九間波羅 (Amatitlania nigrofasciata) 均屬於中型慈鯛科魚類，因獨創性與新穎性高，預估通過生物安全評估後屆時國外市場將達1 千 6 百萬美元產值。由此可知，台灣基因轉殖螢光觀賞魚在全球觀賞魚產業已奠定成熟基礎，並且已展現可預期的經濟價值，具有潛在龐大經濟商機。然而因全球目前對基因轉殖生物安全具有疑慮，憂心一旦基因轉殖魚類流放至野外時，將導致生態平衡的破壞與基因流佈(gene flow)等問題，因此造成基因轉殖觀賞魚行銷受限，而觀賞魚業者也擔心一旦基因轉殖觀賞魚遭人私下繁殖，將造成價格低落導致行銷的困難，因此開發魚類不孕控制技術為拓展觀賞魚產業發展的核心技術。基因轉殖魚類的不孕控制研究是一個重要的生物安全性考量因子，且須為可誘導性方能將基因轉殖魚類傳承下來。本計劃擬利用Piwi 基因表現在初始生殖細胞(PGC)及雌雄生殖細胞精細胞與卵細胞發育過程，而AMH 基因表現在生殖細胞周圍的體細胞精巢之Sertoli 細胞與卵巢之濾泡細胞。擬建立魚類性腺之生殖細胞專一性Piwi1 (或Vasa)啟動子與體細胞專一性AMH (或igf3)基因啟動子表現NTR-EGFP 或KillerRed 誘導性毒性蛋白之基因轉殖斑馬魚及神仙魚，藉由處理基質Mtz 或光照分別破壞初始生殖細胞及性腺之生殖細胞與周圍的體細胞Sertoli 細胞或濾泡細胞以達到完全不孕之目標。本計畫有助於加速基因轉殖螢光斑馬魚及神仙魚通過生物安全評估，讓技術領先與創新的基因轉殖中型螢光觀賞魚早日進入國際市場。" "Ornamental fish industry is a fine aquaculture industry. It is also one of priority subjects of agriculture biotechnology in Taiwan. Since 2009, the first transgenic green and red fluorescent angle fish (Pterophyllum scalare) and cichlid (Amatitlania nigrofasciata) were established by Academia-Industry Cooperation of Academia Sinica, Fisheries Research Institute, National Taiwan Ocean University and Kaohsiung Marine University and Jy Lin Company in Taiwan, thus Taiwan possess the technical leadership in the industry of transgenic ornamental fish. Transgenic fluorescent angle fish and cichlid belong to medium-sized cichlid with originality and novelty. It was expected that the market will reach 16 million while obtaining approval of biosafety assessment. In fact, Taiwan has established a mature foundation of transgenic fluorescent ornamental fish in the global ornamental fish industry, and demonstrated potentially with a hung economic value. However, because of the international concern in genetically modified (GM) fish which may cause gene-flow into the wild population, the global sale of transgenic fish is restricted; moreover the company also worries about the impediment of sale for transgenic ornamental fish due to the private breeding, which will cause the price falling down. For these reasons, the development of infertile control technology is considered to be the core technology for ornamental fish industry requirements. The technology of infertile control for transgenic fish is considered to be prominent issue for meet the GMO biosafety and this technology must be inducible; otherwise the transgenic fish can’t be inherited. In this project, we will establish transgenic zebrafish and angelfish lines, which express an inducible toxin protein nitroreductase-EGFP fusion protein or KillerRed in the primordial germ cells, different developmental stages of germ cells including spermatogonia and oogonia by Piwi1 (or Vasa) promoter or/and in gonad surrounding somatic cells including Sertoli cells and follicle cells by AMH (or igf3) promoter identified from zebrafish, tilapia or angelfish. The cytotoxin will destroy germ cells and surrounding somatic cells of gonads in both male and female transgenic fish and results in infertility by the treatment of Mtz substrate solution or light irradiation. We expect to perform the infertility of transgenic fluorescent zebrafish and angelfish to achieve approval of biosafety assessment and facilitate the middle-sized transgenic fluorescent ornamental fish created by innovation and leading techniques of Taiwan’s research team, to swim into the international markets in the nearby future. "

Keyword(s)

基因轉殖觀賞魚
不孕控制
Piwi
抗穆勒氏賀爾蒙
transgenic ornamental fish
infertility control
AMH
igf3

DSpace CRIS

Development of Infertility Technology in Transgenic Fluorescent Ornamental Fish (II)

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