從生物矽稻殼灰製備成多功能的中孔二氧化矽奈米粒子做為藥物輸送系統

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基本資料

Project title

Code/計畫編號

MOST104-2113-M019-002

Translated Name/計畫中文名

從生物矽稻殼灰製備成多功能的中孔二氧化矽奈米粒子做為藥物輸送系統

Project Coordinator/計畫主持人

Hsiu-Mei Lin

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=11484588

Year

2015

Start date/計畫起

01-08-2015

Expected Completion/計畫迄

31-07-2016

Bugetid/研究經費

950千元

ResearchField/研究領域

化學
生物技術(理)

"因為稻殼裡面有很豐富的二氧化矽，而稻殼是一種便宜的農業廢棄物，含有大量非晶相的水合二氧化矽顆粒。然而，一直沒有人嘗試利用稻米殼中大量的非晶相二氧化矽轉變成中孔洞二氧化矽奈米粒子(MSN)，這邊我們利用稻殼來合成用於生物成像之中孔二氧化矽奈米粒子。奈米粒子顯影劑因在生醫成像及治療診斷學上的多種應用而備受重視。近年來，科學家們對於以多功能二氧化矽奈米粒子(mesoporous silica nanoparticles, MSN)在成像及治療上的雙效應用展現出明顯的研究興趣。這個領域迅速擴展，產生出一個新的術語：「治療診斷學」（syn: theranostic)，這個術語是用來形容作為診斷和治療劑的雙重角色的平台。與傳統慣用的顯影劑相比，二氧化矽奈米粒子擁有多種潛在優勢，此奈米粒子能夠經由設計合成出多功能二氧化矽奈米粒子，希望改善細胞内檢測的靈敏度且能增強標靶的效率。在此項研究中，我們將葉酸、適體和細胞控制修飾到具有雙重顯影功能的奈米粒子，使此中孔二氧化矽奈米粒子同時具有治療與診斷多功能，希望此多功能的二氧化矽奈米粒子應用在生醫成像及治療診斷學，這種組合的多功能二氧化矽奈米粒子能夠同時在患部累積足夠的劑量當作顯影劑及治療藥劑，使它們在未來治療診斷學有相當良好的前景。主要主題包括： a.使用稻殼來合成中孔洞二氧化矽奈米粒子，然後我們在rMSNs中摻雜Eu3+及Gd3+，用來合成擁有螢光及磁性雙重成像功能的EuGd-rMSN，應用於生物成像。 b.通過不同的連接體，將AS1411適體接上雙重顯影奈米粒子EuGd-rMSN的合成出具有雙重顯影-標靶雙功能的EuGd-rMSN-A奈米粒子。 c.將葉酸分子嫁接到EuGd-rMSN-A的表面得到EuGd-rMSN-A-FA奈米粒子。藉由EDC-NHS偶聯來將葉酸標靶接上EuGd-rMSN-A奈米粒子，得到EuGd-rMSN-A-FA雙重顯影-雙標靶雙功能的奈米粒子。 d.最後，在EuGd-rMSN-A-FA奈米粒子上接上細胞内控制藥物(SSCPT)。根據我們之前的研究經驗，藉由優化條件來製備出集結了雙重成像、雙重標靶和細胞控制釋放三種效能合一的的多功能EuGd-rMSN-A-FA-SSCPT奈米粒子。此外，我們將對所有不同的奈米粒子之物理和化學性質進行了研究，以進行體外測試（in vitro)和體内（in vivo)測試。" "Due to the abundance of silica in rice husk, which is a cheap agro-based waste material, which harbors a substantial amount of silica in the form of amorphous hydrated silica grains. However, there have been no attempts at harnessing the enormous amount of amorphous silica present in rice husk transformation into mesoporous silica nanoparticles. We exploited it for the synthesis of a mesoporous silica nanoparticles for biological imaging. The use of nanoparticles imaging agents in diagnosis and therapy is receiving considerable attention in recent years, the research teams involving physicists and material chemists are interest in the multifunctional mesoporous silica nanoparticles (MSNs) because of the dual-model for imaging and therapy. This field is expanding rapidly, bringing a new term: theranostic, which means a combination of therapeutics and diagnostics. Comparing with the traditional imaging agent, MSNs have many advanced features to develop into a multifunctional platform by elaborate design. In order to improve the sensitivity of cellular examination and the effect of targeting, the multifunctional MSNs are going to combine dual imaging, folate, aptamer and cellular control modified to contain the ability of therapy and diagnosis to apply for theranostic biological field. This complex multifunctional MSNs as an imaging and therapeutic agent can accumulate a sufficient amount at lesion, with great prospects of theranostic for the future. The main topic include: 3+ A. Mesoporous silica nanoparticles are going to be synthesized.by rice husk(rMSNs). Then, we dope Eu and Gd3+ into the rMSNs to synthesize the EuGd-rMSN with the fluorescence and magnesium dual imaging function for biological imaging B. A EuGd-rMSN, dual imaging nanoparticles, conjugated with aptamer via different linker was synthesized and characterized. C. The folate molecular is going to graft on the surface of EuGd-rMSN-A to get EuGd-rMSN-A-FA nanoparticles. Dual targeting was achieved by attaching folic acid and the AS1411 aptamer through EDC-NHS coupling. D. Finally, conjugating the intracellular-controlled drug(SSCPT) on EuGd-rMSN-A-FA nanoparticles.. According to our research experience, this multifunctional nano-platform could bring the dual imaging, dual targeting and cellular control together by optimal conditions to prepare the multifunctional EuGd-rMSN-A-FA-SSCPT nanoparticles. Furthermore, the physical and chemical properties of all different nanoparticles were investigated to test in vitro and in vitro assessment."

Keyword(s)

適體標靶
葉酸標靶
癌症治療
藥物控制釋放
智慧型藥物遞送系統
細胞内控制藥物遞送系統
雙重顯影
標靶治療
斷治療奈米粒子
in vitro
in vivo
Aptamer targeting
Folate targeting
Cancer Therapy
Drug Controlled Release
Intelligent Drug Delivery
Intracellular-controled Drug Delivery System
Dual Imaging
Targeting Therapy
Theranostic Nanoparticles

DSpace CRIS

從生物矽稻殼灰製備成多功能的中孔二氧化矽奈米粒子做為藥物輸送系統

基本資料

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