Functions of Hypoxia-Inducible Factors in Zebrafish Embryo (I)

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Project title

Code/計畫編號

NSC92-2313-B019-043

Translated Name/計畫中文名

斑馬魚低氧誘發蛋白基因在胚胎發育時期之功能(I)

Project Coordinator/計畫主持人

Chin-Hwa Hu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=849734

Year

2003

Start date/計畫起

01-08-2003

Expected Completion/計畫迄

01-07-2004

Bugetid/研究經費

1318千元

ResearchField/研究領域

生物技術(理)
生物科學

本計畫於第一年度主要工作在探討低氧誘發蛋白HIF-1α與HIF-3α對於頭骨與顱面的發育影響。首先利用軟骨染色的方法觀察HIF-1α與HIF-3α基因knockdown對胚胎頭骨的影響，結果顯示軟骨細胞排列紊亂，無法成型。隨後利用標識基因追蹤各胚層的發育情況，發現HIF-1α與HIF-3α基因knockdown會造成內中胚層均受到嚴重的影響，造成內胚層所衍生的咽弧，咽囊構造，以及中胚層所發育的顏面肌肉等均有明顯的缺損現象，而頭骨的先驅神經脊細胞的遷移亦受到影響，以致於軟骨的構造排列凌亂，並且大量減少的現象。此外，菱腦的分節亦有緊縮的現象。我們的實驗結果，顯示缺氧細胞訊息對於正常胚胎發育扮演有相當重要的功能。這項工作將持續追蹤較早期的胚胎發育狀況，以及探討腦部重要器官如下視丘，腦下垂體等的影響。 The function of Hypoxia-Inducible Factors, HIF-1a and HIF-3a, in head skeleton and cranial facial development was investigated. Gene-specific morpholino oligonucleotide was injected into fertilized zebrafish embryos at 1-cell stage to observe the effect of gene-knockdown in embryonic development. It appeared that the craniofacial development was disrupted in hif-1a & hif-3a-knockdown embryo. A number of endodermic and mesodermic markers were also blocked in these embryos. Consequently, the pharyngeal pouches and cranial muscle was defected in hif-1a & hif-3a-knockdown embryo. In addition, the neural crest cells migration and segmentation of rhombomere was disturbed by hif-1a & hif-3a morpholino oligonucleotides. These observations suggest that the HIF-1a and HIF-3a-related signaling pathways have important roles in vertebrate development. We will continue to work on this project and investigate the function of HIF-1a and HIF-3a-related signaling in brain development.

Keyword(s)

Hypoxia-inducible factor
Cranial skeleton
Morpholino oligonucleotide

DSpace CRIS

Functions of Hypoxia-Inducible Factors in Zebrafish Embryo (I)

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