Development of Laser Desorption/Ionization-Based Biochips

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Project title

Code/計畫編號

NSC102-2113-M019-001-MY3

Translated Name/計畫中文名

開發雷射脫附游離奈米生物晶片

Project Coordinator/計畫主持人

Chih-Ching Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=8117181

Year

2014

Start date/計畫起

01-08-2014

Expected Completion/計畫迄

31-07-2015

Bugetid/研究經費

1449千元

ResearchField/研究領域

化學
生物技術(理)

"本計畫之目標是結合結合奈米晶片與雷射脫附游離檢測技術於高通量生物晶片之開發。我們將以綠色化學原則合成一系列不同大小和形狀金屬、金屬氧化物和半導體材料奈米粒子。當這些奈米材料塗覆於玻璃或聚二甲基矽氧烷基材後，再將辨識分子修飾上生物辨識分子即可製備奈米晶片。我們將調控辨識分子於晶片上方向性與密度來增加與目標分子的多重結合能力。當奈米晶片與目標生物分子(腫瘤蛋白質、DNA、 RNA 或醣類)、病原體或腫瘤細胞後，藉由脈衝雷射脫附游離所得金屬團簇(雷射脫附游離奈米粒子表面原子)訊號改變即可定量樣品中分析物。我們所開發高通量和免標記的奈米晶片期待能運用於臨床醫學上腫瘤標誌物與基因檢測、病原菌篩選和腫瘤細胞的快速辨識。" The first goal of this proposal is integrating nanochip and laser desorption/ionization (LDI) techniques in the development of novel biochips. First, we will focus on the preparation of metallic, metal oxide and semiconductor nanoparticles (NPs) with different sizes and shapes using green chemical synthetic routes. After coating the NPs on the glass or polydimethylsiloxane (PDMS) substrate, the biorecognition molecules (proteins or single-stranded DNA) will be modified on the nanochips for the preparation of biochips. The orientation, density and distance of recognition units on the chips will be carefully controlled for improving the multivalent binding ability and specificity. We will monitor the signal changes of metallic cluster ions from nanochip under LDI in the absence and presence of targeting molecules (tumor markers, DNA, RNA or polysaccharides), pathogens and tumor cells for the quantification of biomolecules and cells in samples. This technique has a great potential to be applied in the clinical detection of tumor markers, analysis of gene, pathogen screening and recognition of tumor cells in the future.

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Development of Laser Desorption/Ionization-Based Biochips

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