Development of a Fast Analysis Platform for Detecting Specific Gene Using Functional Gold Nanoparticles

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基本資料

Project title

Code/計畫編號

MOST104-2622-M019-001-CC2

Translated Name/計畫中文名

利用功能化金奈米粒子於快速基因檢測平台開發

Project Coordinator/計畫主持人

Chih-Ching Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=11605913

Year

2015

Start date/計畫起

01-11-2015

Expected Completion/計畫迄

31-10-2016

Bugetid/研究經費

588千元

ResearchField/研究領域

化學

"基因檢測於臨床醫學上能提供相當重要的指標，目前主要的檢測方法為利用即時定量聚合酶連鎖反應儀(Real time Quantitative Polymerase Chain Reaction Machine)進行試驗分析，雖然具有高靈敏度但耗時且成本較高，未能滿足高效且低成本的臨床檢測需求。因此本計劃主要目的是開發功能性核酸修飾之金奈米粒子，利用金奈米粒子與腺嘌呤有高度親和力的特性，以尾端為聚腺嘌呤之核酸修飾於金奈米粒子，相較於傳統使用琉醇基修飾核酸於金奈米粒子，成本約可節省5倍以上。結合聚合酶連鎖反應儀擴增目標基因片段後，藉由修飾有核酸之金奈米粒子能專一性與目標基因互補雜交而造成金奈米粒子聚集的原理，可於可見光波段(〜520 nm)檢測目標基因。此方法提供一快速基因檢測平台，能短時間進行基因檢測，並具定量功能，相較於傳統檢測法能更貼近臨床醫學上之簡單、快速、低成本、高靈敏度和專一性的需求，並期望此研究開發快速基因檢測平台可應用於病毒檢測、曱基化基因分析或其它癌症基因分析。 " "Gene detection is the one of important diagnostic tests that can provide useful information to understand the fitness or illness of organs and systems, which can provide early detection of serious diseases. The current method for analysis of DNA is through amplification and quantification of DNA by quantitative polymerase chain reaction (qPCR), which provide very sensitivity and accuraty analysis for target DNA. Although qPCR method has these advancements, the high cost of qPCR instrument limits its wide application. In this proposal, we will develop a high sensitive, high throughput and low cost analysis platform for analysis of specific DNA by employing noncovalent DNA-modified-gold nanoparticles (probe DNA-Au NPs). Considering the disadvantage of the high cost of using thiolated DNA to modify on Au NPs surface, we will use DNA which contain poly-adenine (poly A) tail to prepare probe DNA-Au NPs by the high affinity of poly-adenine and Au NPs. That can develop a cost-effective probe and reduce the cost to 20%. The simple and rapid colormatric assay will be achieved by target DNA-induced aggregation of probe DNA-Au NPs when the target DNA is hybridized with probe DNA. As a result, the concentration can be quantitated by monitoring the absorption at 〜520 nm. This method can provide a rapid, sensitive, highly specific and high throughput analysis platform for detecting DNA. These advantages meet the requirments for the clinical diagnosis, and broader application than qPCR method becauseof their low cost. We believe this analysis platform will become a potential tool of DNA detection in commercial clinical diagnosis. "

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Development of a Fast Analysis Platform for Detecting Specific Gene Using Functional Gold Nanoparticles

基本資料

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