The Application of Chitosan in the Novel Controlled Release Drug Delivery Systems of Antibiotics and Insulin

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基本資料

Project title

Code/計畫編號

NSC91-2313-B019-022

Translated Name/計畫中文名

幾丁聚醣在抗生素與胰島素之新控制釋出輸藥系統上之應用研究

Project Coordinator/計畫主持人

Ke-Liang Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=757816

Year

2002

Start date/計畫起

01-08-2002

Expected Completion/計畫迄

01-07-2003

Bugetid/研究經費

800千元

ResearchField/研究領域

化學工程
藥學
生物科學

本計畫利用幾丁聚醣、果膠及不同交聯劑（三聚磷酸鹽、戊二醛及 genipin）形成複合物顆粒，以製造一種於生物體內特定部位釋放之控制釋放載體，並比較不同交聯劑所製成的幾丁聚醣—果膠顆粒包覆抗生素之效果及其在控制釋放特性上的差異。經烘乾之幾丁聚醣—果膠顆粒粒徑較小，顏色較深，凍乾粒徑較大，顏色較淺。 amoxicillin 在 37℃在 pH 7.4 之緩衝溶液中比其在 pH 1.4 緩衝溶液中有較好的安定性，其降解過程大致呈現一級反應。以 genipin 及 glutaraldehyde 交聯的顆粒較能保護幾丁聚醣在 pH 1.4 酸性環境中不易被溶解。在 pH 7.4 環境下， genipin 及 glutaraldehyde 交聯之幾丁聚醣—果膠顆粒比 TPP 交聯與無交聯之顆粒較能維持恆定的膨潤比。在包覆率的部分，以幾丁聚醣—果膠顆粒約為 61％，以 genipin 交聯之顆粒約為 57％，戊二醛交聯之顆粒約為 51％， TPP 交聯之顆粒約為 49％。藥物釋放而言，四種顆粒於 pH 1.4 下， amoxicillin 抗生素之釋放量很少，均為 6％以下。而在 pH 7.4 下經過 720 分鐘抗生素之釋放量分別為 TPP 交聯顆粒釋放 65％，幾丁聚醣—果膠顆粒釋放 57％，戊二醛交聯顆粒釋放 19％， genipin 交聯顆粒釋放 14％。添加果膠分解酵素後抗生素之釋放比未添加酵素時，增加 1.5∼3.5 倍，在 720 分鐘後之釋放量分別為 TPP 交聯顆粒釋放 93％，幾丁聚醣—果膠顆粒釋放 90 ％，戊二醛交聯顆粒釋放 69％， genipin 交聯顆粒釋放 21％。本研究之結果證實可用不同交聯劑調整幾丁聚醣膠粒之結構及水分 /溶質之擴散速率，進而控制抗生素之釋出速率。 This study investigated the preparation of coacervated and cross-linked chitosan- pectin particles, and measured the release behavior of amoxicillin. The average diameter of air-dried chitosan-pectin particles is smaller than that of the freeze-dried samples and their appearance is darker than freeze-dried particles. The stability of amoxicillin at pH 7.4 was better than at pH 1.4. The degradation of amoxicillin obeyed first-order kinetics. In pH 1.4 environment, genipin and glutaraldehye crosslinked chitosan-pectin particles slowly dissolved. Genipin and glutaraldehye crosslinked chitosan-pectin particles swelled less at pH 7.4. The drug loaing of chitosan-pectin particles, genipin crosslinked, glutaraldehye crosslinked and TPP crosslinked particles was 61%, 57%, 51% and 49% respectively. The release of amoxicillin in pH 1.4 buffer was lower than 6%. The release of amoxicillin in pH 7.4 buffer after 720 min from TPP crosslinked, chitosan-pectin, glutaraldehye crosslinked and genipin crosslinked particles was 65%, 57%, 19% and 14% respectively. The release of amoxicillin in pH 7.4 buffer (in the presence of pectinase) after 720 min from TPP crosslinked, chitosan-pectin, glutaraldehye crosslinked and genipin crosslinked particles was 93%, 90%, 69% and 21% respectively.

Keyword(s)

幾丁聚醣
果膠
抗生素
交聯
Chitosan
Glutaldehyde
Pectin
Cross-linking
Amoxicillin

DSpace CRIS

The Application of Chitosan in the Novel Controlled Release Drug Delivery Systems of Antibiotics and Insulin

基本資料

Description