The Amelioration Effects of Nanoencapsulated Polysaccharides from Antrodia Cinnamomea on Male Reproductive Function in Streptozotocin-Nicotinamide Induced Diabetes

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Project title

Code/計畫編號

MOST105-2313-B019-007

Translated Name/計畫中文名

牛樟芝多醣微奈米粒對STZ誘發糖尿病雄性大鼠生殖功能之保護作用

Project Coordinator/計畫主持人

Zwe-Ling Kong

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=11893014

Year

2016

Start date/計畫起

01-08-2016

Expected Completion/計畫迄

01-07-2017

Bugetid/研究經費

900千元

ResearchField/研究領域

生物技術(農)

本研究計晝為「牛樟芝多醣微奈米粒對STZ誘發糖尿病雄性大鼠生殖功能之保護作用」。探討牛樟芝多醣微奈米粒的雄性生殖保護作用，解決因糖尿病併發的發炎性傷害問題，對代謝症候群的罹患者及生育期群體極為重要，預期將可積極改善生殖健康。近年國内外針對本議題之研究愈趨重視，主持人曾以不同來源牛樟芝多醣及多種保健素材進行相關研究，並結合微奈米包覆強化其保護效果，結果顯示此為安全有效的加工製備方式。本計晝擬分三年期間聚焦於動物口服實驗的組織器官、血液生化值及細胞基因表現活性的變化分析上，實驗材料採用皿培式牛樟芝多醣並製備成氧化矽-幾丁聚醣微奈米粒，以利未來產業發展的技術智財保護與法規依循。研究方法以國際間主流的糖尿病誘發雄性大鼠生殖傷害為模式，雖因動物品系與STZ誘發糖尿病常有實驗失誤，但因累绩多年經驗已經克服。第一年: 選用皿培式牛樟芝多醣、製備成氧化矽-幾丁聚醣微奈米粒、物化特性與安定性分析、動物口服預備實驗之劑量分組設計。第二年:牛樟芝多醣微奈米粒之動物口服實驗、不同劑量投與之功效反應檢討。第三年:牛樟芝多醣奈米粒之最適劑量化、探討吸收分布、代謝路徑與代謝基因體表現研究。 The research theme is- The Amelioration Effects of Nanoencapsulated Polysaccharides from Antrodia cinnamomea on Male Reproductive Function in Streptozotocin-Nicotinamide induced Diabetes. In order to solve the adverse effects caused by inflammatory damage of diabetes mellitus/ metabolic syndrome, we will discussed the protential functions of encapsulated A. cinnamomea polysaccharide micro-nano particles. It is very important issue to those suffering population as well as the natalist, and we are expected to aggressively improve the reproductive health. In recent years, domestic and international research on this subject became into emerging and important. In our study, A. cinnamomea polysaccharides from different sources and a variety of nutraceutic ingredients, combined with micro-nano-coated strengthen its protective effect. Our finding showed that this is a safe and effective method for preparation and processing. During the three years of the project, we intends to focus on animal experiments via oral administration. Including tissues and organs, blood biochemistry values and cellular gene expression wil be conducted. The Dish-cultured A. cinnamomea polysaccharides are selected as the experimental materials, and the silicon oxide polysaccharide-chitosan nanoparticles are prepared. Those designs will facilitate the future development due to intelligence protection and meet the industrial regulations. For methods, the male reproductive damage induced in diabetic rats as a international mainstream model. We have been overcome and skillfull in experience for many years, although both animal strains and STZ-induced diabetes quite tricky. First year: preparation of encapsulated A. cinnamomea polysaccharide micro-nano particles, including the physicochemical properties and stability will be also analysed. Preliminary experiments for the oral administrated dosage by animal grouping. Second year: Animal experiments for encapsulated A. cinnamomea polysaccharide micro-nano particles. The different dosage response according with their effectiveness will be overall reviewed. Third year: Determination of the optimal dosage of encapsulated A. cinnamomea polysaccharide micro-nano particles. Both the uptaking/absorption/distribution/metabolic pathway and the genomic expressing performance wil be elucidated.

Keyword(s)

牛樟芝
奈米包覆
糖尿病
生殖功能
Antrodia cinnamomea
Nanoencapsulation
Diabetes
Reproduction

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The Amelioration Effects of Nanoencapsulated Polysaccharides from Antrodia Cinnamomea on Male Reproductive Function in Streptozotocin-Nicotinamide Induced Diabetes

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