Repeated-Batch Fermentation of Acid and Enzymatic Hydrolysates of Red Alga Gracilaria for Lactic Acid Production Using Microtube Array Membrane-Immobilized Lactic Acid Bacteria

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基本資料

Project title

Code/計畫編號

MOST106-2221-E019-065

Translated Name/計畫中文名

微管束陣列膜固定化乳酸菌重複批次發酵紅藻龍鬚菜酸水解液及酵素水解液生產乳酸

Project Coordinator/計畫主持人

Hong-Ting Lin

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=12272134

Year

2017

Start date/計畫起

01-08-2017

Expected Completion/計畫迄

01-07-2018

Bugetid/研究經費

788千元

ResearchField/研究領域

食品科技(工)

"聚乳酸具有生物相容性、生物降解性等特性，是目前最熱門的高分子聚合物材料之一。乳酸為最主要製備聚乳酸的原料，並廣泛被應用在工業上如製藥及食品業等。據估計，到2020 年，乳酸全球需求量將達到100 萬公噸，而微生物發酵為目前主要乳酸的生產方法。海藻含有高量海藻多醣以及海藻纖維且不影響糧食供應、雨林及耕地，可做為良好的乳酸發酵碳源。目前我國主要養殖的海藻以龍鬚菜較具規模，是台灣唯一可以在陸地上大量養殖之大型海藻。在發酵方式中，利用固定化細胞於重複分批發酵為一種可有效的降低生產成本及提高發酵速度的方式。聚乳酸微管束陣列膜為一種新穎的細胞固定化技術，具備可大規模量產、可回收、成本低及安定性高等優點。故本計畫擬利用聚乳酸微管束陣列膜固定化乳酸菌用於重複批次發酵紅藻龍鬚菜水解液製備乳酸。第一年期將製備紅藻龍鬚菜酸水解液，並探討添加營養物質如脂肪酸對乳酸發酵的影響，最後再以固定化乳酸菌以批次及重複批次發酵龍鬚菜酸水解液製備乳酸。第二年期將製備紅藻龍鬚菜酵素水解液，並探討製備龍鬚菜酵素水解液之最佳糖化酵素組合及酵素使用量，最後再以固定化乳酸菌以批次及重複批次發酵龍鬚菜酵素水解液來製備乳酸。" "Polylactic acid is one of the most popular polymer materials due to its biocompatible, biodegradable and other characteristics. Lactic acid is not only the main raw material for the preparation of polylactic acid, but widely used in industry such as pharmaceutical and food industries. It is estimated that by 2020, global demand for lactic acid will reach 1 million tonnes, and microbial fermentation is the main approach for lactic acid production. Seaweed, which contains high amount of polysaccharides and does not affect the food supply, forests and arable land, can be used as a good carbon source for lactic acid fermentation. At present, red alga Gracilaria is the only alga to be cultivated in large scale and land-based in Taiwan. Among the fermentation methods, the use of immobilized cells in repeated batch fermentation can effectively reduce production costs and improve the speed of fermentation. Poly-l-lactic acid microtube array membrane, a novel cell immobilization technology, has several advantages, such as large scale production, recoverability, low cost and stability. Therefore, this proposal intends to use poly-l-lactic acid microtube array immobilized lactic acid bacteria for repeated-batch fermentation of red alga Gracilaria for lactic acid production. The first year of research will focus on preparation of acid hydrolysate of red alga Gracilaria, and other extra nutrients, such as lipids, to be added for better lactic acid fermentation will be investigated. Then Poly-l-lactic acid microtube array membrane immobilized lactic acid bacteria will be applied in batch and repeated-batch fermentation of Gracilaria acid hydrolysate for lactic acid production. In the second year we aim to optimize the best saccharification enzymes and their optimal loadings for the preparation of enzymatic hydrolysate of Gracilaria sp. Then Poly-l-lactic acid microtube array membrane immobilized lactic acid bacteria will be applied in batch and repeated-batch fermentation of Gracilaria enzymatic hydrolysate for lactic acid production."

DSpace CRIS

Repeated-Batch Fermentation of Acid and Enzymatic Hydrolysates of Red Alga Gracilaria for Lactic Acid Production Using Microtube Array Membrane-Immobilized Lactic Acid Bacteria

基本資料

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