The Development of Production Technology on Biobutanol from Gracilaria Sp.

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Project title

Code/計畫編號

MOST104-ET-E019-001-ET

Translated Name/計畫中文名

龍鬚菜生質丁醇生產技術研發

Project Coordinator/計畫主持人

Chorng-Liang Pan

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=11282345

Year

2015

Start date/計畫起

01-01-2015

Expected Completion/計畫迄

01-12-2015

Bugetid/研究經費

958千元

ResearchField/研究領域

生物技術(理)
能源工程

本研究計畫之目的為研發自紅藻龍鬚菜(Gracilaria sp.) 生產生質丁醇的發酵技術。主要工作包括將龍鬚菜或龍鬚菜熱萃多醣以3-5 組多醣分解酵素降解為可供產丁醇微生物代謝利用之單醣產物，同時探討數項關鍵反應條件如水解液之溫度與pH 值等對於可發酵糖產率的影響，並用HPLC協助確認水解液中寡醣與單醣之組成與含量。龍鬚菜多醣水解液即利用產丁醇菌株擬以批式與饋料批式發酵技術探討發酵中之重要影響因子，如發酵液中之可發酵醣類之組成與濃度、發酵溫度、pH 值、種入之產丁醇菌株菌量與菌齡、鹽濃度、以及重要中間產物丁酸等之變化。並擬利用氣體回收的技術提升丁醇的發酵產率。以及瞭解到達發酵終點時發酵液中之醣類與醇類等之組成與含量，做為未來持續提升自各類海藻生產生質丁醇的研發基礎。並以生命週期評估(LCA) 的方法瞭解產製龍鬚菜生質丁醇之生產成本與所產生之環境效益(對降低碳排放量之正面影響)。本年度研究計畫期望能達成自紅藻龍鬚菜經水解發酵產出8-10%生質丁醇的研發目標。 The objective of this study is to develop potential fermentation techniques for producing bio-butanol from green algae Gracilaria sp. Gracilaria sp. and its hot water extracted polysaccharides (PS) are target Experimental Materials (EM) for the following enzymatic digestion. Three to five variables of PS digesting enzyme mixtures degrade EM to obtain fermentable simple sugars (FSS), which can be further metabolized and fermented to butanol by microorganism. Several key factors for optimal FSS yield such as digesting temperature and pH value are evaluated. Amount and composition of oligosaccharides and monosaccharides from Gracilaria hydrolysates are analyzed by HPLC. Utilizing batch type and fed batch type of fermentation, hydrolysate of Gracilaria sp. is fermented by butanol producing bacteria is mainly assessed on critical production factors such as yield of FSS, fermentation temperature, pH value, amount and age of starters, salt concentration, and the change of butyric acid. In addition, gas striping technique is applied to increase the fermentation yield of bio-butanol, and composition and concentration of carbohydrates and alcohols from fermented substance in the end of reaction are expected as meaningful research foundation for future developing bio-butanol production from various algae species. The changes of cost and carbon footprint of different bio-butanol production procedure from Gracilaria sp. are evaluated by Life Cycle Assessment (LCA) method, and these results could be used to lower down both production cost and CO2 releasing amount. The study goal of this project is to produce 8-10 g butanol/100 g of Gracilaria sp. via hydrolysis and fermentation process.

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The Development of Production Technology on Biobutanol from Gracilaria Sp.

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