Improvement on the Efficiency of Fucoxanthin Extraction from Laminaria Japonica Assisted by Multiple Enzymes and Development on the Application of Seaweed Residues through Biorefinery Process(1/2)

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簡歷

基本資料

Project title

Code/計畫編號

MOST108-2221-E019-042-MY2

Translated Name/計畫中文名

海帶經多重酵素輔助生產褐藻素之萃取效率提升及開發藻渣之生物精煉利用(1/2)

Project Coordinator/計畫主持人

Chorng-Liang Pan

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13120648

Year

2019

Start date/計畫起

01-08-2019

Expected Completion/計畫迄

31-07-2020

Bugetid/研究經費

397千元

ResearchField/研究領域

食品科技(工)

本研究目的為開發多重酵素輔助萃取褐藻素之方法，以提升萃取褐藻素之產率，並探討海帶之生物精煉製程，生產出海帶鹽、低分子量海帶寡醣、乳酸、及海帶藻渣等生物化合物。首先利用本實驗篩得之二株海洋細菌 Pseudomonas vesicularis MA103 與 Aeromonas salmonicida MAEF108 所誘導出多種酵素降解海帶細胞壁多醣，再以乙醇萃取海帶之褐藻素 (Fucoxanthin)，最後以膠體過濾層析對褐藻素進行純化，並分析其體外腸道益生性及抗氧化效果。另一方面，為了減少藻渣廢棄物之產生，本研究亦探討海帶可能之生物精煉製程，利用漂洗、超過濾系統區分、及乳酸發酵等加工技術，生產海帶鹽、低分子量海帶寡醣、及乳酸等生物化合物。最後將製程終點之海帶藻渣進行一般成分及單醣組成之分析，評估其可能做為動物飼料添加物之潛力。 This research is aim to develop the approach of extracting fucoxanthin assisted by multiple enzymes that increasing the yield of fucoxanthin from Laminaria japonica. Moreover, this research also studies on the biorefinery process of Laminaria japonica to produce the biochemical materials: seaweed salt, low molecular weight of Laminaria oligosaccharides, lactic acid, and Laminaria residues. Firstly, two marine bacteria Pseudomonas vesicularis MA103 (MA103) and Aeromonas salmonicida MAEF108 (MAEF108) selected by our lab can be induced for producing multiple enzymes to degrade the cell wall polysaccharide of Laminaria. Further, the fucoxanthin is extracted by ethanol from Laminaria which processed by the enzymes. After ethanol extraction, the next step is followed by purification via gel filtration chromatography. The fucoxanthin is analyzed by bioactivity experiments to investigate the effect of prebiotics and anti-oxidant activity. In addition, to reduce the seaweed wastes production, this research also studies on the possible processes, such as using washing, ultrafiltration, and lactic acid fermentation, to produce biochemical materials: seaweed salt, low molecular weight of Laminaria oligosaccharides, and lactic acid. Finally, for evaluating the potential application of Laminaria residues which occurred at the end of process on animal feed additive, the Laminaria residues is analyzed by the proximate analysis and sugar composition.

Keyword(s)

海帶
褐藻素
多重酵素輔助萃取
生物精煉
Laminaria japonica
Fucoxanthin
Multiple Enzymes Assisted Extraction
Biorefinery

DSpace CRIS

Improvement on the Efficiency of Fucoxanthin Extraction from Laminaria Japonica Assisted by Multiple Enzymes and Development on the Application of Seaweed Residues through Biorefinery Process(1/2)

基本資料

Description