pH Sensitive Curcumin-Loaded Chitosan/Fucoidan Nanoparticles against Colon Cancer

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簡歷

基本資料

Project title

Code/計畫編號

NSC102-2221-E019-004

Translated Name/計畫中文名

pH值敏感型幾丁聚醣/褐藻糖膠奈米粒攜帶薑黃素對抗大腸癌的研究

Project Coordinator/計畫主持人

Yi-Cheng Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=3119058

Year

2013

Start date/計畫起

01-08-2013

Expected Completion/計畫迄

01-07-2014

Bugetid/研究經費

782千元

ResearchField/研究領域

醫學工程

"本計畫書擬以幾丁聚醣(Chitosan)與褐藻糖膠(Fucoidan)製備 pH 值敏感型奈米粒，攜帶及釋放薑黃素(Curcumin)，探討其對抗腸癌的效果。申請者藉 100年度及 101年度的國科會補助計畫：從幾丁聚醣奈米粒釋放溶疤酶及生長因子應用於脊髓半斷靈長類動物之研究(NSC98-2320-B-019-001-MY3)及新穎性褐藻糖膠奈米粒釋放基質細胞衍生因子，應用於幹細胞療法(NSC 101-2221-E-019 -026)，得到令人雀躍的結果，已發表在 Acta Biomaterialia及 Carbohydrate polymers等國際期刊，後續的實驗仍持續進行，成果亦持續發表中。過去一系列的研究著重於幾丁聚醣的應用，幾丁聚醣取自於海洋，因而想探討其他海洋資源在生醫材料利用的潛力，並結合藥物傳輸系統與食品科學的概念，協助癌症的治療。薑黃素為天然機能性食品成份，能有效抑制腸癌等多種癌症，為極具潛力之抗癌成份，然而其穩定性及水溶性不佳，通過腸胃道後常因被降解而導致生物利用率明顯降低，因此，希望藉由口服載體攜帶及控制釋放薑黃素，克服其使用上的限制並提高生物利用率，以增加其應用的價值。褐藻糖膠(Fucoidan)為萃取自褐藻的生物性多醣類，含有帶負電之硫酸基團，經研究指出具有抗發炎、抗腫瘤、抗病毒、調控生長因子等生理活性；幾丁聚醣(Chitosan)為帶正電的生物聚合物，已知具有多種生物活性與良好的生物相容性。本研究將以褐藻糖膠與幾丁聚醣製備 pH值敏感型奈米載體，攜帶及控制釋放薑黃素，應用於口服傳輸系統，以穩定薑黃素並提高其在水溶液中的含量，再透過體外細胞實驗、SD (Sprague Dawley)大鼠的動物實驗模式及極致液相層析串聯式質譜儀(UPLC-MS/MS)分析系統的建立，評估薑黃素在動物體內的生物利用率，達到有效之抗癌效果。此外，亦期待對於海洋資源的開發及利用，提供一個新的方向。 " "The plan is intended to fabricate chitosan/fucoidan pH sensitive nanoparticles as carriers to deliver curcumin for colon cancer therapy. By the National Science Council grant program (Sustained release of chondroitinase and growth factors from chitosan nanoparticles for repairing the hemisection spinal cord injured primate, NSC98-2320-B-019-001-MY3 and Delivery of stromal cell derived factor-1 from novel fucoidan nanoparticles in stem cell therapy, NSC 101-2221-E-019 -026), we got exciting results which have been published in the international journal “Acta Biomaterialia” and “Carbohydrate Polymers”. Follow-up experiments are still ongoing, and the results continue to publish. Series studies in the past focused on the application of chitosan which comes from ocean. Therefore, I would like to explore other marine resources for the feasibility of biomaterials. Then, combine marine resources with the concept of controlled release system and food science to assist cancer therapy. Curcumin (Cur), a polyphenolic compound extracted from turmeric (Curcuma longa), is a kind of natural functional food and has anti-tumor activities. However, its extremely low solubility and poor stability lead to poor oral bioavailability. In addition, curcumin is easily degraded by enzymes when passing through gastrointestinal digestive tract. Therefore, oral delivery system that carry and controlled release curcumin can overcome the limitation of curcuimin and improve its oral bioavailability. Fucoidan, extracted from brown algae, is a polysaccharide with sulfated groups and has bioactivities including anti-inflammatory, anti-virus, anti-tumor, and anticoagulant. Chitosan, a cationic biopolymer, has known with good bioactivity and biocompatibility. The aim of this project is to design pH sensitive chitosan/fucoidan nanoparticles for oral delivery system to carry and controlled release of curcumin. Try to stabilize curcumin through gastrointestinal digestive tract and increase its solubility in buffer solution. By in vitro cell tests and the establishment of SD (Sprague Dawley) animal model and UPLC-MS/MS analyzing system, we could evaluate the oral bioavailability of curcumin in vivo. We expect that this oral delivery release system could achieve the purpose of assist colon cancer therapy and provide a new direction for the development and use of marine resources. "

Keyword(s)

褐藻糖膠
幾丁聚醣
pH值敏感
奈米粒
薑黃素
生物利用率
Fucoidan
Chitosan
pH-sensitive
Nanoparticle
Curcumin
Bioavailability

DSpace CRIS

pH Sensitive Curcumin-Loaded Chitosan/Fucoidan Nanoparticles against Colon Cancer

基本資料

Description