Composition and Characteristics of Fouling Fungi on Fishing Vessels (III)

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簡歷

基本資料

Project title

Code/計畫編號

NSC88-2313-B019-005-A22

Translated Name/計畫中文名

台灣北部海域漁船初期附著生物之組成---漁船附著真菌之組成及特性之探討(III)

Project Coordinator/計畫主持人

Guo-Jane Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=434404

Year

1999

Start date/計畫起

01-08-1998

Expected Completion/計畫迄

01-07-1999

Bugetid/研究經費

500千元

ResearchField/研究領域

漁業
生物科學

本研究探討細菌及酵母菌彼此對其附著之影響,以及菌體與材料片表面疏水性對酵母菌附著力之影響。當海水懸吊片之酵母菌分離株Candida guilliermondii與細菌分離株Pseudomonas cepacia共同存在時,對彼此附著力影響不大。然而若材料片表面先有細菌附著,明顯降低隨後酵母菌之附著力;反之,酵母菌之預先附著並不影響隨後細菌之附著。分別以HIC (Hydrophobic Interaction Chromatography) 及MATH (Microbial Adhesion to Hydrocarbon) 方法測定酵母菌表面疏水性,前者因酵母菌體太大不易通過膠體孔隙而不適用。以MATH分析4株海水懸吊片分離株C. guilliermondii, Debaryomyces polymorphus,Candida humicola及Rhodotorula rubra在brain heart infusion broth (BHIB)生長時之表面疏水性變化,前二者隨菌齡增加而增大,附著力亦隨之增大;後二者之表面疏水性及附著力無相關性。在海水中,酵母菌表面疏水性,隨菌齡增加明顯下降,然附著力卻隨之增加。4株酵母菌對不同表面疏水性之5種材料片glass,steel,teflon,polythylene及polystyrene之海水系統之附著力,顯示不論菌體或材料片表面疏水性均非決定其附著力之因子。 The aims of this research are to investigate the interaction of bacteria and yeast, and the effects of the surface hydrophobicity of yeast and solid surface on the adhesive ability in seawater. The co-existence of bacterial isolate, Pseudomonas cepacia,and yeast isolate, Candida guilliermondii did not affect the adhesive ability for each other. However, the pre-adherence of bacterium reduced the adhesive ability of yeast. On the contrary, the pre-adherence of yeast did not affect bacterial attachment. The cell surface hydrophobicity of yeasts was evaluated by the methods of hydrophobic interaction chromatography (HIC) and microbial adhesion to hydrocarbon (MATH). HIC method was not suitable because some yeast cells were too large to pass through the resion gap. The cell surface hydrophobicity of 4 yeast isolates, C. guilliermondii, Debaryomyces polymorphus, Candida humicola and Rhodotorula rubra varied with age. In seawater, cell surface hydrophobicity decreased, and adhesion value increased with the in creasing of cell age. Neither cell surface hydrophobicity nor solid surface hydrophobicity affected the adhesion value of yeasts, based on the results of the attachment of yeasts on the surfaces of the substrata of glass, steel, teflon, polyethylene and polystyrene in seawater.

Keyword(s)

真菌
附著生物
抗真菌附著
表面特性
漁船
Fungi
Biofouling
Anti-fungal fouling
Surface characteristic
Fishing boat

DSpace CRIS

Composition and Characteristics of Fouling Fungi on Fishing Vessels (III)

基本資料

Description