Effects of Chitooligosaccharides on the Animal Intestinal Bacteria

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簡歷

基本資料

Project title

Code/計畫編號

DOH91-TD-1013

Translated Name/計畫中文名

幾丁寡醣對腸內細菌之影響

Project Coordinator/計畫主持人

Guo-Jane Tsai

Funding Organization/主管機關

Environmental Protection Administration

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=742327

Year

2002

Start date/計畫起

01-08-2001

Expected Completion/計畫迄

01-07-2002

Bugetid/研究經費

800千元

ResearchField/研究領域

基礎醫學
食品科技(農)

幾丁寡醣乃幾丁聚醣經化學處理或生物處理產生分子斷裂後之產物，其分子大小乃由2∼9個葡萄糖胺分子構成(亦即分子聚合度 DP=2∼9)為水溶性物質。由體外試驗及動物注射得知幾丁寡具有多種功能，包括抗菌活性、活化巨噬細胞、剌激抗體產生、增強對病菌抵抗力、抑制腫瘤當胞生長等。然而，幾丁寡醣分子大小影響其生理活性，目前研究顯示DP≧6之幾丁寡醣方具上述生理功能。幾丁寡醣之製備可以酸或強氧化劑進行分子斷裂，亦可以酵素法進行水解，如何掌控反應條件，以得到具生理活性的幾丁寡醣為生產幾丁寡醣的關鍵。酵素法由於反應條件溫和，再現性好，廣為人們重視。本研究室三年前開始探討以廉價商業酵素生產具生理活性的幾丁寡醣，已篩選得理想酵素，所得寡醣具良好抗菌活性及免疫增強活性，在食品保鮮及保健食品極具開發潛力。已知果寡醣、半乳寡醣等不為人體消化吸收之寡醣產品，具有改善腸內細菌及調節腸道生理之功能。幾丁寡醣不被人體消化吸收，其是否亦具有同樣功能？此值得人們探討。由於幾丁寡醣本身具有抗菌活性，此乃與其它寡醣不同之處。其對細菌之抗菌活性隨菌體不同而異，因此，幾丁寡醣進入動物腸道後，若具有改善腸內細菌之功能，則其所具有的可能機制到底是1.如同其它寡醣般，當做腸內有益菌的營養源，促進其生長，改善腸內菌叢；還是2.因其具抗菌活性，可有效抑制腸內壞菌生長，腸內益菌對其耐性較強，因而改善腸內菌叢？此有待人們釐清。本研究將延續以前的研究，利用已建立的生產條件，以市售酵素生產幾丁寡醣，進行幾丁寡醣的動物餵食試驗，探討其對腸內主要有益菌Lactobacillus與Bifidobacterium及有害菌Clostridium perfringens生長的影響，同時，探討腸道中由細菌所產生之短鏈脂肪酸之變化，另外，探討其對肝功能，腸壁組織細胞之影響，以確認其未來供做健康食品之安全性。 "Chitooligosaccharide, a water-soluble substance, is composed of glucosamine monomer with the degree of polymerization of 2-9 (DP 2-9). It can be prepared via chemical or enzymatic degradation of chitosan, Several tests demonstrate that chitooligosaccharides have many physiological activity, including antimicrobial activity, macrophage activation, stimulating antibody production, increasing resistance to infectious disease and suppressing the growth of tumor cells. However, the molecular size of chitooligosaccharide will affect its bioactivity, with DP≧6 being with bioactivity. The reaction condition is the crucial point for the production of chitooligosaccharides with bioactivity during chemical (acids, or strong oxidant) or enzymatic degradation of chitosan. Recently, people are more interested in enzymatic treatment due to the mild reaction condition and good reproductivity. Three years ago we stared to screen various commercial enzymes in order to produce chitooligosaccharide shows potential in food preservation and functional food. Some oligomers, such as fructooligosaccharide and galactooligosaccharide can not be digested by human. They can improve the intestinal microflora and regulate the physiological function of human. Does chitooligosaccharides have similar function because it is indigestible? In addition, chitooligosaccharides have strong antibacterial activity and this activity is various with different bacteria. Therefore, if chitooligosaccharides can improve the intestinal microflora, what will be its mechanisms: due to being as prebiotics for the growth of probiotics, or due to its selective inhibition of intestinal pathogens? All these unknown questions merit further research. The aims of this research are to produce chitooligosaccharide by enzymatic degradation of shrimp chitosan using established reaction condition, ,to investigate its effects in vitro and in vivo on the intestinal pathogen Clostrigium perfringens, and probiotics such as Lactobacillus and Bifid obacterium, to detect its effect on the changes of short chain fatty acids in intestine, and to evaluate its effects on the intestinal epithelial tissue and liver function for the safety issues of functional food."

Keyword(s)

幾丁寡醣
腸內細菌
腸內益生菌
短鏈脂肪酸
Chitooligosaccharide
Intestinal bacteria
Probiotics
Short chain fatty acid

DSpace CRIS

Effects of Chitooligosaccharides on the Animal Intestinal Bacteria

基本資料

Description