Fermentation of Soybean Meal Using Co-Cultures of Lactic Acid Bacterium and Bacillus Subtilis Natto for Preparation of Feed Nutrient Additive with Anti-Obesity Activity( II )

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簡歷

基本資料

Project title

Code/計畫編號

MOST107-2622-E019-001-CC2

Translated Name/計畫中文名

以乳酸菌與納豆菌複合菌株發酵豆粕開發具抗肥胖活性之飼料營養添加劑( II )

Project Coordinator/計畫主持人

Guo-Jane Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=12427783

Year

2018

Start date/計畫起

01-02-2018

Expected Completion/計畫迄

01-01-2019

Bugetid/研究經費

1076千元

ResearchField/研究領域

食品科技(工)

"豆粕由於蛋白質含量高、價廉且供應穩定, 為重要蛋白質供應者。已知去糖基大豆異黃酮及胜肽具抗肥胖活性。本階段研究以乳酸菌 FPS 2520 及桿菌 N1 為測試菌株，分別將 FPS 2520 與 N1 以單菌株模式發酵豆粕懸浮液，以提高豆粕去糖基異黃酮與氨基酸營養含量，並建立單株發酵最條件以利後續複合菌株實驗探討。FPS 2520 與 N1 之最佳發酵豆粕懸浮液之條件為接種 1% 4 log 菌液 ( v/v) 於豆粕水分比率為 1:8 之懸浮液，並以 500 mL balffed flask 在 37oC 以 120 rpm 震盪發酵 72 小時 (FPS 2520 發酵液先靜置 24 小時，後以相同轉速震盪)，FPS 2520 的發酵產液 Daidzein、Genistein、FAN 與 Total peptide含量分別為 25.8±0.7、336.3±10.1、5355.7±89.1 與 299.8±36.3 mg/L，分別為原始未發酵之豆粕懸浮液三種成份含量的 10.00, 5.74,與 2.20倍。N1的發酵產液 Daidzein、Genistein、FAN 與 Total peptide含量分別為 22.0±1.9、297.9±23.9、9830.0±167.7 與 313.0±17.3 mg/L。結果表明豆粕經由FPS 2520 與 N1菌株發酵能提升去醣基異黃酮含量之含胺基酸營養產物，具備抗肥胖活性之水產飼料營養添加劑之潛力。本計畫經第一年得到之各菌株發酵豆粕條件，及將各不同模式所得發酵產物，以3T3-L1 細胞之抗肥胖活性。第二年將由第一年所得最佳複合菌株、以及FPS2520 或 N1 單株菌之發酵產品，以及未發酵之豆粕為測試樣品，分析其一般組成、胺基酸組成、異黃酮含量、胜肽量、ACE 抑制活性、以及3T3L-1 細胞抗肥胖活性分析及對細胞內HSL 及LPL 活性影響。最後進行肥胖大鼠餵食試驗，探討其抗肥胖功效及可能機轉。" Soybean meal (SBM) has the beneficial effects of high protein content and steady supply that SBM becomes as an alternative for feed protein. Isoflavone aglycosides and soy peptides have been demonstrated to have anti-obesity. The aim of this study is to ferment SBM suspension to increase the contents of isoflavone aglycosides and amino acid for the preparation of feed nutritional additive with anti-obesity potential. Lactobacillus sp. FPS 2520 and Bacillus sp. N1 were used as the tested strains in this study. After incubation of SBM suspension inoculated with single strain of 1% 4 log FPS 2520 or N1 (v/v) and using 500 mL balffed flask and shaking at 120 rpm at 37oC for 72 h, the contents of daidzein, genistein, FAN and total peptides content were 25.8±0.7, 336.3±10.1, 5355.7±89.1 and 299.8±36.3 mg/L; 22.0±1.9, 297.9±23.9 , 9830.0±167.7 and 313.0±17.3 mg/L, respectively. In conclusion, the SBM fermented products by FPS2520 or N1 have high contents of isoflavone aglycosides and amino acid, and show potential for aquatic feed additive with anti-obesity. In the first year, the fermentation conditions for SBM fermentation by the mixed strains of FPS 2520 and N1 were established. The fermenting conditions for SBM by individual strains were evaluated, which will provide the information for the second stage investigation of the conditions for mixed strains fermentation. The preliminary anti-obesity activity of the fermented product will be evaluated using 3T3-L1 adipocytes. In the second year, the analyses of the proximate compositions, amino acid composition, isoflavones and peptide contents, ACE inhibitory activity, and in vitro anti-obesity activity including effects on intracellular HSL and LPL activities in 3T3-L1 adipocytes will be provided on the samples of co-culture fermented product, single culture fermented product and unfermented SBM from the first year. Then, the in vivo anti-obesity activity and proposed mechanism for the tested sample will be investigated by oral feeding of the high fat diet- induced rats.

Keyword(s)

飼料營養添加劑
豆粕
乳酸菌
納豆菌
複合菌發酵
抗肥胖
Feed nutrient additive
Soybean meal
Lactic acid bacterium
Bacillus subtilis natto
mixed culture fermentation
anti-obesity

DSpace CRIS

Fermentation of Soybean Meal Using Co-Cultures of Lactic Acid Bacterium and Bacillus Subtilis Natto for Preparation of Feed Nutrient Additive with Anti-Obesity Activity( II )

基本資料

Description