Microbial Fermentation of Shrimp Shell Waste for the Production of Fermented Shrimp Shell Product with Hypoglycemic Activity

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Project title

Code/計畫編號

MOST109-2221-E019-031

Translated Name/計畫中文名

微生物發酵蝦殼廢棄物生產具降血糖活性的蝦殼發酵產品

Project Coordinator/計畫主持人

Guo-Jane Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13541972

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

1241千元

ResearchField/研究領域

食品科技(工)

蝦殼廢棄物富含蛋白質等營養成分，極易孳生蚊蠅及細菌生長，造成惡臭。然而其含有豐富蛋白質、幾丁質、蝦紅素等，是寶貴保健食品原料。綜觀目前利用微生物發酵蝦殼廢棄物的研究，主要利用微生物產生的有機酸來促使蝦殼中礦物溶出，以及微生物產生的蛋白酶來降解與幾丁質或蝦紅素結合的蛋白質，以利蝦殼中蝦紅素的萃取或幾丁質的分離，目的均只為了蝦紅素或幾丁質單一物質的生產。本計畫前期計畫已從白蝦分離得到135 株幾丁質分解菌及29 株乳酸菌分離菌株，從中篩選出幾丁質分解力及蛋白質分解力強的二株分離菌株LV1111及LV2122，以及乳酸菌LV3204菌株，建立複合菌株發酵技術，以有效分解蝦殼所含蛋白質及幾丁質，釋放出所含礦物質與蝦紅素。本計畫將由前期所建立的複合菌株發酵蝦殼的發酵條件，生產發酵蝦殼產品，將分析其一般組成分及活性成分，後者包含胺基酸組成、胜肽含量、蝦紅素含量、幾丁寡醣或小分子幾丁聚醣組成。由於發酵液含具有降血糖潛力的蝦紅素、活性胜肽、幾丁聚醣或寡醣，故將以小鼠肝臟細胞FL83B細胞模式，探討發酵液之降血糖活性，將探討對細胞吸收葡萄糖活性及肝醣產生的影響、對細胞內葡萄糖代謝相關酵素活性的影響、及對細胞產生葡萄糖輸送蛋白的影響，以了解其作用機轉。最後以streptozotocin及nicotinamide及誘導SD rat成第二型糖尿病大鼠的模式，餵食所得蝦殼發酵產品，確認其降血糖活性。 Microorganisms and insects can quickly proliferate in shrimp shell waste (SSW), which contains high amounts of nutrients including proteins, and accordingly, this will contaminate the environment. On the other side, due to containing chitin, protein, and astaxanthine SSW is a good source for the preparation of healthy food. Till this moment, the major purpose on the topic of microbial SSW fermentation in the published papers was only for the separation of single component of chitin or astaxanthin from SSW. The final aim of this research is to ferment SSW using mixed strains isolated from shrimp to obtain the fermented SSW with multiple bioactivity. In the previous year, 135 strains with chitinolytic activity and 26 strains of lactic acid bacteria (LAB) had been isolated from white shrimp(Litopenaeus vannamei); among which two strains of LV1111 and LV2122 with strong chitinylic and proteolytic activity, and LAB strain LV3204 were screened out. The fermentation conditions by using these mixed strains to ferment SSW had been established in order to efficiently degrade the components of proteins and chitin, and to release astaxanthin and minerals from SSW. In this project, SSW will be fermented using mixed culture fermentation techniques obtained from previous year. The proximate composition, and the amounts of the bioactive substances including free amino acid, peptides, chitooligosaccharides/ water-soluble chitosan, and astaxanthin in the fermented SSW will be analyzed. Due to the presence of astaxanthin, peptide, chitosan/ chitooligosaccharide, which have been demonstrated to have hypoglycemic activity , the hypoglycemic activity of the fermented SSW will be investigated using a mouse liver cell of FL83B cell model. The effects of the fermented product on cell uptake of glucose and glycogen production, on the glucose metabolism related enzyme, and on glucose transporter expression will be investigated. Moreover, the hypoglycemic activity of the fermented SSW will be further investigated by oral feeding experiment using streptozotocin and nicotinamide induced type 2 diabetic SD rats.

Keyword(s)

蝦殼廢棄物
幾丁聚醣
蝦紅素
降血糖活性
混株發酵
Shrimp shell waste
Chitin
Astaxanthin
Hypoglycemic activity
Mixed strains fermentation

DSpace CRIS

Microbial Fermentation of Shrimp Shell Waste for the Production of Fermented Shrimp Shell Product with Hypoglycemic Activity

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