Studies on Accumulation of TTX and PSP in Marine Animals, and Physiological and Biochemical Characteristics and Preventive Treatments of These Toxin (II)

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基本資料

Project title

Code/計畫編號

NSC91-2313-B019-001

Translated Name/計畫中文名

河魨毒、麻痺性貝毒在水產生物中之蓄積機制、生理生化特性和防治對策探討(II)

Project Coordinator/計畫主持人

Deng-Fwu Hwang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=737316

Year

2002

Start date/計畫起

01-08-2002

Expected Completion/計畫迄

01-07-2003

Bugetid/研究經費

1765千元

ResearchField/研究領域

食品科技(農)
漁業

The toxins in the new gastropods Oliva miniacea, O. mustelina and O. nirasei implicated in a food paralytic poisoning incident in South Taiwan in Feburary 2002 were studied. The remaining gastropods were divided into digestive gland and edible portion and determined for toxicity by using tetrodotoxin (TTX) bioassay. It was found that the three species of gastropods contained moderate amounts of toxin in edible portion only, and the highest toxicity score was 18 MU/g for O. miniacea, 10 MU/g for O. mustelina, and 27 MU/g for O. nirasei. The toxin was partially purified from the toxic specimens of each species by ultrafiltration using a YM-1 membrane, followed by chromatography on Bio-Gel P-2 column. Analyses by high performance liquid chromatography, gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry showed that the toxin from O. miniacea, O. nirasei and O. mustelina contained TTX, and related compounds 4-epi TTX and anhydro-TTX. The paralytic shellfish poisons were not found. Hence, the causative agents in these gastropod species implicated in this food poisoning incident were identified as TTX and related substances.

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Studies on Accumulation of TTX and PSP in Marine Animals, and Physiological and Biochemical Characteristics and Preventive Treatments of These Toxin (II)

基本資料

Description