Studies on Species Identification of Raw and Processed Puffer Using Protein Methods and DNA Technique(III)

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Project title

Code/計畫編號

NSC91-2313-B019-003

Translated Name/計畫中文名

蛋白質和基因技術作為河魨魚及其加工品的魚種鑑定方法之探討(III)

Project Coordinator/計畫主持人

Deng-Fwu Hwang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=737319

Year

2002

Start date/計畫起

01-08-2002

Expected Completion/計畫迄

01-07-2003

Bugetid/研究經費

997千元

ResearchField/研究領域

食品科技(農)

以Kocher等學者設計之引子(L 14724與H 15149)，可成功增幅16種蒐集所得之台灣常見河魨之部分Cytochrome b gene片段，其片段長度為486 bp，此組引子增幅之PCR產物經限制脢Bsa JI、Hinf I、Bsa I 、Sap I、Taq I、Eco RV、SspI、Ear I、Stu I、Bsr DI切割後可分成四大步驟來區分；利用此鑑別法之優點除可減少分析時間外亦可降低分析成本，在現今快速檢測需求之壓力下，可提供快速簡便之分析優勢。 Phylogenetic relationships among puffer fish were investigated by comparing cytochrome b gene sequences and restriction endonuclease assays of 16 species from Taiwan. DNA was prepared for sequencing by the PCR. No variation in sequences was detected among individuals within each species. Direct estimates of mitochondrial cytochrome b gene sequence divergence among 16 puffer fish were from 3.41 to 31.78%. Different restriction patterns were found among 16 puffer fish with ten restriction endonucleases, while no variation in patterns was detected among individuals within each species. The polymorphisms obtained by RFLP have provided a new set of genetic markers for the accurate identification of sibling puffer species. It is the first molecularly based study of puffer diversity. It sheds light on the evolution and taxonomy of this major puffer fish family.

Keyword(s)

Phylogeny
Puffer fish
Cytochrome b gene
PCR-RFLP
Species identification
Mitochondrial DNA

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Studies on Species Identification of Raw and Processed Puffer Using Protein Methods and DNA Technique(III)

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