Study on Risk Assessment of Ciguateric Toxins and Excess Vitamin a in Taiwanese Fish( I )

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基本資料

Project title

Code/計畫編號

MOST109-2320-B019-001

Translated Name/計畫中文名

台灣產魚類中熱帶性海魚毒和過量維生素A之風險評估研究(1/3)

Project Coordinator/計畫主持人

Deng-Fwu Hwang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13540428

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

1300千元

ResearchField/研究領域

食品科技(農)

歷年來在台灣海洋生物毒引起之食物中毒原因物質，以河魨毒、麻痺性貝毒、熱帶性海魚毒 (ciguateric toxins) 和過量維生素A引起之食物中毒事件最頻繁，且發生死亡事件，甚為國人所重視。其中熱帶性海魚毒素已知含有5種不同構型的毒素，其中國內外最易發生食物中毒之毒素為西卡毒素和菟葵毒素，且由於地球暖化，西卡毒素引起之食物中毒更加頻繁，最近台灣開發養殖成功之龍虎石斑魚，業者打算外銷至日本時，被要求需做一系列毒性評估資料，日本才願意審查核可。目前本研究室已完成老鼠生物毒性檢測法 (mouse bioasaay) 協助業者建立1年養殖龍虎斑之毒性監測。另外在貴部之目前計畫中，正在執行珊瑚礁大型野生魚類龍膽石斑和老虎石斑之肝臟中西卡毒素及過量維生素A之食用風險評估。引起熱帶性海魚毒中毒之魚類，在台灣常見者有笛鯛類、紅甘鲹類、箱河魨類和鯙魚類，這些毒素之分析目前須依賴高效液相層析儀串聯二次質譜儀才能檢測完成，魚種須賴基因技術才能確認。以往台灣之熱帶性海魚毒中毒，本研究室大多以小白鼠生物檢測法分析，加上應用基因技術鑑定魚種，才建立了中毒個案資訊。因此本研究第一年擬探討曾發生食物中毒之鮨科和紅甘鰺科魚種之基因鑑定技術及可能毒素酵素進行純化種定性含量探討。第二年繼續針對鯛科類和龍占魚科的魚種之基因鑑定技術及可能毒素酵素進行純化定性定量技術探討。第三年擬針對箱河魨科和鯙科魚種之基因鑑定技術及可能毒素菟葵毒或西卡毒進行定性定量分析技術探討。併檢測肝臟中維生素A之含量，以建立台灣海魚類食用上之安全資訊。 Tetrodotoxin (TTX), paralytic shellfish poison (PSP), ciguateric toxins and excess vitamin A are marine toxins in Taiwan which caused many poisoning accidents by ingesting seafoods in recent years. In this study, the ciguateric toxins in fish related to food poisoning will be investigated particularly. The liver of related toxic species from Taiwan will be collected to analyze their toxicity by using mouse bioassay. Their DNA data for species identification is also studied. Moreover, the vitamin A level is also analyzed by HPLC analysis. The risk assessment of seafood safety in ciguateric toxins and excess vitamin A in the liver of big type (over 3 kg) fish will be established. The related fish species include Serranidae, Lutjanidae, Carangidae, Lethrinidae, Sparidae and Muraenidae. This study will be performed for three years. Each year we will determine more than 10 fish species and 100 fish samples. Those data are useful to make sure the safety of seafood.

Keyword(s)

西卡毒素
菟葵毒素
過量維生素 A
魚類
小鼠生物毒性試驗法
高效液相層析儀
高效液相層析儀串聯二次質譜儀
ciguatoxin
vitamin A
coral reef fish
grouper
mouse bioassay
HPLC
LC-MS/MS

DSpace CRIS

Study on Risk Assessment of Ciguateric Toxins and Excess Vitamin a in Taiwanese Fish( I )

基本資料

Description