Phytoplankton Genes Specific to the Ultradian Growth Mode and the Stationary Phase:Gene Identification and Field Applications (II)

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基本資料

Project title

Code/計畫編號

NSC90-2313-B019-017

Translated Name/計畫中文名

浮游藻類快速增殖期及生長停滯期特有基因之篩選與野外應用(II)

Project Coordinator/計畫主持人

Jeng Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=648522

Year

2001

Start date/計畫起

01-08-2001

Expected Completion/計畫迄

01-07-2002

Bugetid/研究經費

1051千元

ResearchField/研究領域

漁業
生物技術(農)

本計劃的長程目標在篩選微細藻類體內與族群生長相關的基因，作為野外浮游植物生長狀態的指標。過去一年中完成的工作是測定周氏扁藻所含的「高親合性磷酸傳輸蛋白」在各種缺磷條件下的表現情形，並證實基因表現量與磷供應量緊密相關。另外也針對一種矽藻進行了篩減型反轉錄-聚合脢連鎖反應，共獲得 4 條與快速生長相關的基因以及 1 條與生長停滯相關的基因，目前正積極測試它們在不同環境下的表現情形。The long term goal of this study is to screen phytoplankton genes that are related to population growth, and to use these genes as markers of phytoplankton growth potential in the sea. During the past year, a gene encoding a high-affinity phosphate transporter in Tetra- selmis chui was characterized, and its expression was tightly associated with the phosphorus availability in the medium. In addition, the subtractive reverse transcription- polymerase chain reaction was performed on a diatom to identify growth-related genes. Using this procedure, we obtained 4 genes specific to the exponential phase of population growth, and 1 gene specific to the stationary phase. We are currently evaluating there expression levels under various growth conditions.

Keyword(s)

基因篩選
生長停滯期
浮游性藻類
野外應用
Gene selection
Stationary phase
Phytoplankton
Field application

DSpace CRIS

Phytoplankton Genes Specific to the Ultradian Growth Mode and the Stationary Phase:Gene Identification and Field Applications (II)

基本資料

Description