Phytoplankton Genes Specific to the Ultradian Growth Mode and the Stationary Phase:Gene Identification and Field Applications (III)

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Project title

Code/計畫編號

NSC91-2313-B019-012

Translated Name/計畫中文名

浮游藻類快速增殖期及生長停滯期特有基因之篩選與野外應用(III)

Project Coordinator/計畫主持人

Jeng Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=758513

Year

2002

Start date/計畫起

01-08-2002

Expected Completion/計畫迄

01-07-2003

Bugetid/研究經費

1064千元

ResearchField/研究領域

生物技術(農)
漁業

本計劃的長程目標在篩選微細藻類體內與族群生長相關的基因，作為野外浮游植物生長狀態的指標。過去三年中完成的工作首先是測定周氏扁藻所含的「高親合性磷酸傳輸蛋白」在各種缺磷條件下的表現情形，並證實基因表現量與磷供應量緊密相關。另外也針對一種矽藻進行了篩減型反轉錄 -聚合脢連鎖反應，共獲得 4 條與快速生長相關的基因 (名稱暫定為 RG#14、 RG#21、 RG#25、及 RG#42) 以及 1 條與生長停滯相關的基因 (名稱暫定為 DS#2)。這些基因均已取得接近全長之序列，並已測試了它們在不同環境下的表現情形。基因 DS#2 的表現量在族群開始死亡時最高，約為接種初期的 500 倍。至於編號為 RG 的 4 條基因均在接種初期生長旺盛時表現量高，而當藻株進入生長緩慢的靜止期後表現量便減少，其中又以 RG#42 表現量對族群生長的變化最為顯著。這些基因的表現量可藉定量反轉錄 -聚合脢連鎖反應在混合藻種的情況下加以測定，也都有在野外實測浮游植物生長狀態的實用價值。 The long-term goal of this study is to screen phytoplankton genes that are related to population growth, and to use these genes as markers to estimate phytoplankton growth potential in the sea. During the past 3 years, a gene encoding a high-affinity phosphate transporter in Tetraselmis chui was characterized, and its expression was tightly associated with the phosphorus availability in the medium. In addition, the subtractive reverse transcription-polymerase chain reaction was performed on a diatom to identify growth-related genes. Using this procedure, we obtained 4 genes (code names: RG#14, RG#21, RG#25, and RG#42) specific to the exponential phase of population growth, and 1 gene (code name: DS#2) specific to the stationary phase. For these genes, full-length sequence analysis was close to its completion, and their expression levels under various growth conditions were evaluated. The expression of DS#2 reached the highest level, about 500 times the level on the second day after inoculation, at the end of incubation when the population entered death phase. In contrast, the 4 genes specific to the rapid-growth (RG) stage showed high expression when new cultures were initiated. As the population growth of algal cells decreased with time, the expression of these genes decreased as well. Among them, RG#42 showed the greatest variation in response to growth rate. It is also confirmed that the expression level of individual genes can be estimated by quantitative reverse transcription-polymerase chain reaction using RNA extracted from a mixture of algal species. Thus, these genes have the potential to become molecular marker for the status of phytoplankton population growth in marine environments.

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Phytoplankton Genes Specific to the Ultradian Growth Mode and the Stationary Phase:Gene Identification and Field Applications (III)

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