The Effects of Nutrient Composition on the Growth and Relative Dominance of Ultra-Phytoplankton (II)

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簡歷

基本資料

Project title

Code/計畫編號

NSC91-2611-M019-016-OP3

Translated Name/計畫中文名

東海長期觀測與研究(I)控制碳通量之環境因子---子計畫II：營養鹽組成、超微浮游植物生長與相對優勢之研究(II)

Project Coordinator/計畫主持人

Jeng Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=769710

Year

2002

Start date/計畫起

01-08-2002

Expected Completion/計畫迄

01-07-2003

Bugetid/研究經費

1018千元

ResearchField/研究領域

海洋科學
生物科學

本研究為一三年期之長期計劃，目標在界定那一種營養鹽會控制超微浮游植物的生長，希望能釐清超微浮游植物與大型浮游植物間的關係，並探討東海海域有機碳的流向及食物鍊的結構。計劃執行期間首要工作為測試流式細胞儀鑑別超微浮游植物的能力與準確度，以便順利處理培養實驗的樣本。在以流式細胞儀計數東海及南海的水樣後，已找出可以清楚辨識聚球藻、原核綠藻、與微微真核藻等超微浮游生物的最佳儀器設定。接著就進行營養鹽添加的培養實驗，對基隆沿岸與東海南部測站的水樣分別添加硝酸鹽、磷酸鹽、金屬離子、與複合維生素。結果顯示只有添加金屬離子的處理能有效刺激聚球藻的生長，間接指出河流輸入與大氣落塵對東海生態系的重要性。 This study is a 3-year research project. The research goal is to identify the critical nutrient species that controls the population growth of ultraphytoplankton in the East China Sea. This limiting nutrient may control the relative dominance between ultraphytoplankton and phytoplankton of larger sizes, which in turn may affects the carbon cycling and food chain structure in the East China Sea. Before nutrient enrichment experiments could be initiated, a newly purchased flow cytometer must be tested to evaluate its performance in identifying various ultraphytoplankton and its accuracy in cell counting. In a series of test runs, the flow cytometer was used to analyze water samples from the South China Sea and the East China Sea. Optimal settings in measurement parameters were confirmed, and Synechococcus, Prochlorococcus, and picoeukaryotes could be reliably identified based on cell size and pigment content. Next, nutrient enrichment experiments were conducted at a coastal station near Keelung City and at 2 stations in the East China Sea. In each experiment, aliquots of water samples were enriched with nitrate, phosphate, metal ions, and vitamins, separately, and cell number of Synechococcus was monitored with flow cytometer during the 36 hrs. incubation period. The result indicated that only the treatment of metal enrichment stimulated the growth of Synechococcus, which revealed the importance of terrestrial runoff and atmospheric dust to the East China Sea ecosystem.

DSpace CRIS

The Effects of Nutrient Composition on the Growth and Relative Dominance of Ultra-Phytoplankton (II)

基本資料

Description