Expression Strategies of Diatom Nitrogen- and Phosphorus-Utilization Genes under the Competitive Stress from Cyanobacteria

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Project title

Code/計畫編號

NSC101-2313-B019-006

Translated Name/計畫中文名

矽藻體內氮磷吸收基因面對藍綠細菌競爭時的因應策略

Project Coordinator/計畫主持人

Jeng Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=2635210

Year

2012

Start date/計畫起

01-08-2012

Expected Completion/計畫迄

01-07-2013

Bugetid/研究經費

950千元

ResearchField/研究領域

海洋科學
生物技術(理)
漁業

"海洋中，矽藻與單細胞藍綠藻競爭營養鹽的優勝劣敗決定了有多少有機碳會被輸送至魚類的食階，所以對評估與預測漁業資源有其重要性。由於傳統的競爭係數在偵測上十分困難，而且會隨著浮游植物的生理狀態改變，本申請案計畫以三年的時間探討矽藻與單細胞藍綠藻競爭營養鹽的分子機制，目標是觀察單細胞藍綠藻如何影響矽藻的基因表現，然後以實驗室的觀察為基礎在東海中確認競爭強度。研究的手段是將屬於矽藻的相關角刺藻和屬於藍綠藻的聚球藻以單獨與共同的方式培養在全營養鹽、缺氮、和缺磷的條件下，再以定量反轉錄聚合脢連鎖反應偵測營養鹽吸收相關基因的表現量，觀察是否受到競爭的影響。接著要進行競爭狀態下的全轉錄體分析，尋找共同培養時才會出現的相關角刺藻基因，並篩選合適的基因作為競爭的指標。為了比較競爭行為的種間差異，也計劃針對第二種矽藻 (肋紋骨藻) 進行類似的實驗。最後將設法至東海採集樣本，以全轉錄體定序觀察競爭指標基因的表現情形。本申請案採用了先進的技術研究具生態意義的議題，預期所產生的結果將有助於建立矽藻營養鹽競爭的分子機制、在種類混雜的海洋樣本中顯示矽藻遭受的競爭壓力、並解釋矽藻在東海中的分佈。" "In marine environment, nutrient competition between diatoms and unicellular cyanobacteria determines the amount of organic carbon transported to the trophic level of fish. Traditionally, competition coefficients are used to express the strength of competition. However, these parameters vary with physiological states of phytoplankton and are difficult to measure. This application proposes a 3-year study plan that aims to investigate the molecular mechanisms of nutrient competition between diatoms and unicellular cyanobacteria. The research goal is to understand how cyanobacteria affect the gene expression in diatoms and to establish a method for the detection of competition in the East China Sea. First, the diatom, Chaetoceros affinis, and the cyanobacteria, Synechococcus WH7803, will be grown either separately or together in a series of growth conditions including nutrient repletion, nitrogen deficiency, and phosphorus deficiency. Quantitative reverse-transcription polymerase chain reaction will be used to measure the expression levels of genes involved in nutrient uptake to determine if they are influenced by the competition stress. Next, transcriptomes will be constructed for C. affinis that is grown together with Synechococcus. This approach will lead to a broad search for all genes that express differentially under nutrient competition, and suitable indicator genes of competition status will be screened. A second species of diatoms (Skeletonema costatum) will be similarly measured to evaluate species-to-species variations in response to competition. Finally, samples will be collected from the East China Sea for the construction of a meta-transcriptomic databank. By the identification of the homologs of competition indicator genes, the existence of competition pressure in natural assemblages of diatoms can be examined. This proposal employed advanced techniques to study an ecologically important topic. Experimental results generated by this project will reveal molecular mechanisms of nutrient competition in diatoms and will be helpful to explain the distribution of diatoms in the East China Sea. In addition, techniques developed by this research will be capable of detecting competition pressure in samples containing a mixture of various species."

Keyword(s)

浮游植物
矽藻
單細胞藍綠藻
營養鹽
種間競爭
指標基因
全轉錄體
diatoms
indicator genes
interspecific competition
nutrients
phytoplankton
transcriptome
unicellular cyanobacteria

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Expression Strategies of Diatom Nitrogen- and Phosphorus-Utilization Genes under the Competitive Stress from Cyanobacteria

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