Effects of River Discharge and Nutricline Depth on Diatom Nucleic Acid Content and Population Growth

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Project title

Code/計畫編號

MOST104-2611-M019-009-MY3

Translated Name/計畫中文名

全球變遷對西北太平洋臺灣海域海洋生物地球化學與生態系統影響之長期觀測與研究(Ⅱ)-子計畫：河川輸出與營養鹽躍層對矽藻核酸含量和族群生長之影響

Project Coordinator/計畫主持人

Jeng Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=12034201

Year

2017

Start date/計畫起

01-08-2017

Expected Completion/計畫迄

01-07-2018

Bugetid/研究經費

1960千元

ResearchField/研究領域

海洋科學

"本申請案為整合型計畫「全球變遷對西北太平洋臺灣海域海洋生物地球化學與生態系統影響之長期觀測與研究（二）」中的子計畫，計畫緣起是基於全球變遷使得東海面臨海洋層化和河川輸出優養化等改變，於是研究矽藻的「硝酸鹽傳輸蛋白基因」Nrt2，藉由基因的mRNA 表現量來顯示主要藻種的生長是否受到含氮營養鹽的限制，在第一期整合型計畫中已得到數次在東海的測量成果。在第二期的整合型計畫中，申請人希望延續以往之成果，繼續在東海南部對Nrt2 的表現量進行長期觀測，並在重點測站以次世代定序技術建構全轉錄體，做為確認目標矽藻Nrt2 序列的依據。另外增加rRNA:rDNA 比例的測量項目，著眼點是快速增殖的細胞均富含rRNA，所以這項比例可輔助解釋Nrt2 表現量的測量結果。最後會將測量結果和水文參數進行比較分析，在馬祖沿岸海域觀察河川輸出的效應，而在臺灣海峽到黑潮一帶觀察營養鹽躍層深度的影響。本申請案與其他子計畫間有密切的合作與互動，所需的水文資料和營養鹽濃度將來自「基礎生產力變動」子計畫，而所得營養鹽限制資料也能匯入現有的生態模式，配合其他子計畫共同評估東海生態系在環境變遷時的反應。" "This proposal is a branch project under the integrated research project “Effects of Global Change on Ocean Biogeochemistry and Ecosystems in the Seas surrounding Taiwan in the Northwest Pacific (II)” (ECOBEST-II). The original motivation was that the East China Sea (ECS) would face enhanced stratification and altered river discharge under the general trend of global change. Since these changes are nutrient-related, the study of diatom nitrate transporter gene, Nrt2, may reveal the status of nitrogen deficiency in dominant diatoms via mRNA abundances of Nrt2. This approach has generated encouraging results in the first phase of ECOBEST. In the second phase, the principal investigator plans to continue the measurement of Nrt2 expression in the southern ECS. Next generation sequencing technology will be used to construct transcriptomes at selected stations to confirm gene sequences in dominant diatoms. In addition, in light of fast proliferating cells having a higher cellular content of ribosomal RNA, the rRNA:rDNA ratios will be measured for ECS samples. This ratio should complement the results of Nrt2 expression in determining the severity of nitrogen stress. The measured Nrt2 expression levels will be analyzed with respect to hydrographic parameters. The effects of river discharge will be studied in the coastal area off the Matsu Islands, and the nutricline effects will be studied alone the cross-shelf track from Taiwan Strait to Kuroshio. This proposal will work collaboratively with other branch projects under ECOBEST-II. Hydrographic information and nutrient concentrations will be provided by the “Variations in primary productivity” branch project. The mechanisms of nutrient stress obtained by this proposal will be fed to an existing ecological model of ECS in order to achieve a comprehensive understanding of environmental changes."

Keyword(s)

東海
全球變遷
海洋層化
河川輸出
浮游植物
營養鹽
營養鹽躍層
指標基因
全轉錄體
核酸比例
East China Sea
global change
marine stratification
river discharge
phytoplankton
nutrients
nutrient stress
indicator gene
transcriptome
DNA ratio

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Effects of River Discharge and Nutricline Depth on Diatom Nucleic Acid Content and Population Growth

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