Study of Silica Nanoparticle Drug Delivery System with Marine Characteristic

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簡歷

基本資料

Project title

Code/計畫編號

MOST105-2119-M019-002

Translated Name/計畫中文名

具海洋特色之二氧化矽奈米粒子藥物輸送系統研究

Project Coordinator/計畫主持人

Hsiu-Mei Lin

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=11887436

Year

2016

Start date/計畫起

01-08-2016

Expected Completion/計畫迄

01-07-2017

Bugetid/研究經費

900千元

ResearchField/研究領域

化學

在人類所有的疾病中，癌症的死亡率一直都偏高，而常用的癌症化療藥物都有很高的副作用，因此，研發具有安全性、標靶性及具控制釋放的奈米藥物載體和萃取具有治療癌症的生物活性物質，成為治療癌症的新方向。矽藻二氧化矽奈米粒子(DSNs)與中孔洞二氧化矽奈粒子(MSNs)都具有孔洞結構，可以載入藥物。它們都表面具有矽醇基，十分容易進行官能化修飾(例如標靶修飾及加上控制釋放系統）。本研究利用矽藻對稀土金屬離子的代謝作用，培養具有螢光及磁性性質的矽藻。將螢光及磁性性質的矽藻矽藻經過去除有機質及物理性處理後，製得具有螢光及磁性性質的矽藻二氧化矽奈米粒子(EuGd-DSNs)。在 EuGd-DSN表面官能化修飾上適體(aptamer)標靶分子AS1411後，把褐藻酶膠(fucoidan)載入 EuGd-DSN。褐藻醣膠是一種具有抗癌功效的生物活性物質，它的主要萃取自海洋大型藻類的褐藻。最後，使用雙硫鍵(S-S)官能化修飾的方法，在載入褐藻醣膠的DSN的孔洞加上控制釋放系統當守門員。本研究主要分為四個部分:（i)製備具有磁共振顯影及光學顯影特性之DSN，(ii)接上標靶，（iii)載入褐藻糖膠，（iv)最後加上控制釋放系統。 Mortality rate of cancer has always been high due to the negative side effects of commonly used cancer chemotherapeutic drugs. Therefore, research for a pharmaceutical nanocarrier that safely targets and deliver novel compounds is of great importance in the direction for the treatment of cancer. Diatom silica nanoparticles (DSNs) have pore structures that can facilitate the loading of novel compounds. Their surface have silanol groups that can be easily modified to function for target modifications as well as a controlled drug delivery release system. In this research, diatom that has fluorescent and magnetic property is cultured by the metabolic activity of the rare earth metal ion. Fluorescent and magnetic properties of diatomaceous silica nanoparticles (EuGd-DSNs) are first prepared by adding organic solvent to remove its organic matter and then by physical processing of this diatom. The aptamer target ligand is frst attached to EuGd-DSN surface membrance. Fucoidan- a biologically active substance with anti-cancer properties extracted from marine macroscopic brown algae is then loaded within the pore structure of EuGD-DSNs. Finally, the addition of a ‘gatekeeper5 on the loaded-Fucoidan-DSN pore. The gatekeeper utilizes the method of disulfide bonds (S-S) functionalized modification, acting as a controlled release systems upon cell entry. This study is mainly divided into four parts: (i) preparation of DSN that have magnetic resonance and fluorescent imaging property, (ii) grafting aptamer ligand on to cell surface of DSN, (iii) loading Fucoidan, and finally (iv) the addition of the drug control release system.

Keyword(s)

矽藻土
矽藻土二氧化矽奈米粒子
雙標靶
藥物控制釋放
diatomite
diatomite silica nanoparticle
dual target
control release system

DSpace CRIS

Study of Silica Nanoparticle Drug Delivery System with Marine Characteristic

基本資料

Description