Immune Response and Molecular Biodefense Mechanism in Penaeid Shrimps under Environmental Stress

瀏覽統計 Email 通知 RSS Feed

簡歷

基本資料

Project title

Code/計畫編號

NSC95-2313-B019-013-MY3

Translated Name/計畫中文名

對蝦在環境緊迫下的免疫反應及分子防禦機制

Project Coordinator/計畫主持人

Jiann-Chu Chen

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1590491

Year

2008

Start date/計畫起

01-08-2008

Expected Completion/計畫迄

31-07-2009

Bugetid/研究經費

1621千元

ResearchField/研究領域

漁業

白蝦、草蝦及斑節蝦為台灣目前重要的海水養殖蝦種。近17 年來，海水蝦(對蝦屬)遭受包括孤菌（如Vibrio harveyi、V. alginolyticus）及病毒（如monodon baculovirus 及white spot syndrome virus）感染，導致台灣海水蝦養殖產量逐年減少。在集約式養殖系統中，蝦常會遭遇到物理-化學因子：溫度、鹽度、溶氧及pH；也常遭受到代謝產物如氨、亞硝酸、硝酸、硫化物、二氧化碳及甲烷的增加而影響生理及免疫反應狀況。此外，茶粕、四級胺、次氯酸、地特松、硫酸銅及高錳酸鉀等為養殖池中常用的治療劑，此等物質殘留也影響蝦之生理及免疫反應狀況。疾病的爆發與環境、宿主及病原間有著密切的關係。環境因子變動影響甲殼類的生理及免疫力及對病原的抵抗力。藉由監測總血球數、不同形態血球數、酚氧化酵素活性、超氧離子產生、吞噬能力及清除能力來評估蝦的免疫力及防禦能力。本計劃基於過去在蝦的免疫因子反應，及蝦對於弧菌的抵抗力，以及免疫相關蛋白基因研究已建立的基礎下，繼續研究環境緊迫影響下蝦的免疫力，對於細菌的免疫抵抗，以及研究免疫相關蛋白質（LGBP、βGBP、proPO、 peroxincetin、serine proteinase、α2-macroglobulin、SOD、lysozyme 及 penaeidin）在環境緊迫下的基因及蛋白質表現。本計畫擬在第一年，研究基礎水溫、溶氧、鹽度及pH 變動下，對於蝦免疫反應，對於弧菌抵抗力（水溫、鹽度除外），以及蝦免疫相關蛋白質的基因及蛋白質的表現模式；第二年研究代謝產物，如二氧化碳、硫化物、氨及硝酸對於對蝦免疫反應，對於弧菌的抵抗力（氨除外），以及免疫相關蛋白質的基因及蛋白質的表現模式；第三年研究養殖池常用治療劑，包括茶粕、地特松、次氯酸、及四級胺對於對蝦類免疫反應，及對於弧菌的抵抗力，以及免疫相關蛋白質的基因及蛋白質的表現情形。 Tiger shrimp Penaeus monodon, kuruma shrimp Marsupenaeus japonicus, and white shrimp Litopenaeus vannamei are the major species currently being cultured in Taiwan and other regions in the world. However, since 1988 shrimp culture business has suffered serious problems linked to infectious disease due to vibriosis caused by Vibrio harveyi and V. alginolyticus, and several viruses such as MBV (monodon baculovirus) and WSSV (white spot syndrome virus) resulting in vast economic losses. In an intensive culture system, penaeid shrimps are under the influence of numerous physico-chemical changes like temperature, salinity, dissolved oxygen and pH. In intensive ponds, ammonia, nitrite, nitrate, sulfide, carbon dioxide, and methane are the common metabolites. In addition, several therapeutants like copper sulfate, potassium permanganate, saponin, trichlorfon, malchlite green, hypochlorite and benzalkonium chloride are commonly used in pond water management. The interactions of three factors: deteriorated environment, weakening host and pathogen aggression are required for an occurrence of disease outbreak. Change in environmental parameters influence the immune system of decapod crustaceans. Hemocyte counts including THC (total hemocute count) and DHC (differential hemocyte count), phenoloxidase activity, release of superoxide anion, superoxide dismutase activity (SOD), phagocytic activity and clearance efficiency against pathogen are the common parameters used to evaluate the immune ability of invertebrates. In addition, several proteins including 1) pattern recognition proteins (LBP, LGBP, βGBP, PRGP, lectin), 2) proteins involved in prophenloxidase activating system (proPO, peroxinectin, serine proteinase, pacifascin and a2-macroglobulin), 3) enzymes involved in phagocytosis and productions of reactive oxygen intermediates like SOD and peroxidase, 4) proteins involved in clotting systems (clottable protein and transglutaminase), 5) anti-microbial pepeides like penaeidin and 6) anti-bacterial protein-lysozyme participate in important immune functions of penaeid shrimps. This proposal is to study immune parameters of shrimp and its susceptibility against Vibrio (except temperature and salinity), and gene and protein expression level of immune involved proteins, when shrimp are subjected to physico-chemical stress like temperature, salinity, dissolved oxygen and pH during the first year. This proposal is to study the immune parameters of shrimp and its susceptibility against Vibrio (except ammonia and nitrite), gene and protein expression level of immune involved proteins, when shrimp are under the stress of sulfide, carbon dioxide, ammonia, nitrate and nitrite during the second year. This proposal is to study immune parameters of shrimp and its susceptibility against Vibrio, and gene and protein expression level of immune involved proteins, when shrimp are exposed to different concentrations of thrapeutantss like saponin, trichlorfon, hypochlorite and benzalkonium chloride during the third year. Concerning the immune involved proteins: gene and protein expression and their expression pattern, we plan to study LGBP, βGBP, prophenoloxidase, peroxinectin, serine proteinase, a2-macroglobulin, SOD, penaeidin and lysozyme.

Keyword(s)

白蝦
草蝦
斑節蝦
物理-化學因子
水溫
溶氧
鹽度
pH
低溶氧
代謝產物
二氧化碳
硫化物
氨
硝酸
治療劑
茶粕
地特松
次氯酸
四級胺
免疫力
免疫抵抗
基因表現
蛋白質表現
表現模式
White shrimp
Tiger shrimp
Kuruma shrimp
Physico-chemical factors
Temperature,Salinity
Dissolved oxygen
pH
Metabolites
Sulfide
Carbon dioxide
Ammonia,Nitrate
Nitrite
Therapeutants
Saponin
Trichlorfon
Hypochlorite
Benzalkoniumchloride

DSpace CRIS

Immune Response and Molecular Biodefense Mechanism in Penaeid Shrimps under Environmental Stress

基本資料

Description