Experimental Animal Domestication of Cuttlefish, Squid, and Octopus and It'S Genetic Management-2

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Project title

Code/計畫編號

MOST109-2313-B019-004

Translated Name/計畫中文名

烏賊、鎖管及章魚之實驗動物馴化及其遺傳管理-2

Project Coordinator/計畫主持人

Jung-Hua Wang

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

徐德華
潘彥儒

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=13541968

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

1150千元

ResearchField/研究領域

漁業

頭足類是世界重要的商業漁業之一。在過去15年，台灣烏賊、鎖管及章魚的年捕獲量急劇下降，資源出現匱乏的徵兆。在實驗動物方面，頭足類已成為世界重要的研究對象，但國內實驗室僅能從野外取得，極度不穩定。因此我們將進行烏賊、鎖管（萊氏擬烏賊）及章魚（真蛸）的繁養殖開發、實驗動物馴化，及其種原管理等。本研究為台灣唯一同時具有（1）烏賊規模化養殖培育技術、（2）連續五年辦理資源放流（3）具分子標記技術，可進行種原管理之團隊。我們每年培育烏賊受精卵至少3萬顆、放流種苗至少5000尾，對漁業管理、水產養殖有重要貢獻。在養殖培育技術方面，我們將針對頭足類的體色、個體互動、攝食行為及成熟速度等進行研究，並嘗試馴化為全人工飼料。在分子標記方面，我們將虎斑烏賊、金烏賊、唇瓣烏賊、日本無針烏賊、萊氏擬烏賊、真蛸等物種之轉錄組，整合至基因組資料庫中。並針對各物種的通用及特殊標記進行開發。我們希望發揮場域特色及養殖技術優勢，探討養殖與野生頭足類的行為差異，並改善其養殖及放流效益。透過四年期的計畫，進行頭足類長期繼代培養及實驗動物馴化，達到解決資源復育、水產養殖及科學研究的目標。 The cephalopods are commercially important fisheries worldwide. However, in recent years, the cuttlefish, squid, and octopus are threatened in Taiwan. The annual capture production has dramatically decreased in the past 15 years. Also, Cephalopods has become an important research object in the world in recent years, but standard laboratories can only be obtained from the wild, extremely unstable. Therefore, we would like to develop cephalopods breeding and seedling production technology, Experimental animal domestication of cuttlefish, squid, and octopus and it's genetic management. We are the particular case of large-scale breeding, releasing, and genetic management of cuttlefish in Taiwan. We cultivate at least 30,000 fertilized eggs and 5,000 seedlings per year for stock enhancement for the last five years, which have significant contributions to fishery management and aquaculture. For breeding technology, we will study the body color, individual interaction, feeding behavior, and maturation time of cephalopods, and try to domesticate them to accept artificial feed. For molecular markers, we will integrate the transcriptomes of species such as Sepia pharaonis, Sepia esculenta, Sepia lycidas, Sepiella japonica, Sepioteuthis lessoniana, and Octopus vulgaris into the database. It also develops common and special microsatellites for these species. Through this project, we will complete the domestication of three cephalopod experimental animals and explore the behavioral differences between cultured cephalopods and wild cephalopods, and further optimize and improve the benefits of cephalopod breeding and release.

Keyword(s)

頭足類
實驗動物
微衛星標記
資源放流
水產養殖
cephalopods
experimental animal
microsatellite
stock enhancement
aquaculture

DSpace CRIS

Experimental Animal Domestication of Cuttlefish, Squid, and Octopus and It'S Genetic Management-2

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