The Study of Phagotrophic Mechanisms in Mixotrophic Picoeukaryotes in the Subtropical Northwestern Pacific( I )

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Project title

Code/計畫編號

MOST109-2611-M019-018

Translated Name/計畫中文名

副熱帶西北太平洋混營性超微真核生物的攝食機制探討(1/3)

Project Coordinator/計畫主持人

Yun-Chi Lin

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=13562514

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

847千元

ResearchField/研究領域

海洋科學

Mixotrophic picoeukaryotes serve as important primary producers and also bacterivores in subtropical oligotrophic systems. Mixotrophy is important in nutrient and carbon cycling, however, it has been generally neglected in ecological models. Mixotrophic picoeukaryotes are mainly comprised of haptophytes, cryptophytes, dinoflagellates, chlorophytes, however, little is known about their community composition and phagocytosis mechanisms in the subtropical northwestern Pacific. In this project, we will study these topics by conducting the cruises of East China Sea and North Pacific Subtropical Gyres (Sail to Blue Sea). Firstly, the community composition of mixotrophic picoeukaryotes will be revealed by sequencing of 18S rRNA V4 region. Second, the genes involved in phagotrophy will be studied by grazing experiments and RNA-Seq analysis. This project will advance our understanding on ecologic roles of mixotrophic picoeukaryotes in microbial loop of subtropical ecosystem. 在副熱帶寡營養鹽海域中混營性超微真核生物是重要的基礎生產者也是嗜菌者。混營性在營養鹽與碳循環中是相當重要的，但是在生態模式中卻總是被忽略。目前所知的混營性超微真核生物主要包含有著鞭毛藻、隱鞭毛藻、渦鞭毛藻與綠藻等，但是目前對於它們在副熱帶西北太平洋的種類組成與攝食機制並不清楚。本計畫會利用東海海域與北太平洋環流(航向藍海計畫)的航次來研究混營性超微真核生物的種類組成與攝食機制。首先利用定序18S rRNA V4區段來釐清種類組成，之後利用攝食實驗與基因表現量來研究與嗜菌相關的基因。本計劃有助於了解混營性真核生物在副熱帶生態系的微生物環中所扮演之生態角色。

Keyword(s)

微生物環
混營
超微真核生物
嗜菌
原生生物
RNA定序
Microbial loop
Mixotrophy
Picoeukaryotes
Phagotrophic
Protists
RNA-seq

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The Study of Phagotrophic Mechanisms in Mixotrophic Picoeukaryotes in the Subtropical Northwestern Pacific( I )

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