The Aerobic Exercise-Induced Epigenetic Adaptations in Cardiomyocytes(I)

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Project title

Code/計畫編號

MOST109-2314-B182-026-MY3

Translated Name/計畫中文名

有氧運動誘發心肌細胞的表觀遺傳適應(I)

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

許智欽(計畫主持人)
王鐘賢
傅鐵城
洪明銳

Department/Unit

School of Traditional Chinese Medicine, Chang Gung University

Website

https://www.grb.gov.tw/search/planDetail?id=13537947

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Co-Investigator(s)

Pang-Hung Hsu

Bugetid/研究經費

1090千元

ResearchField/研究領域

復健醫學

我們假設，適當的運動會改善血行力學，透過基因甲基化影響心肌細胞的表觀遺傳適應，活化心肌細胞，進而延長運動改善心肌收縮能力的時間，最終提升健康人或心臟病患的存活率。為了證實這個假設，我們將於未來逐年完成下列幾個目標。第一年：主要在建立運用健康人在不同強度的急性運動前、後的血清培養心肌細胞的方法學；第二年：利用第一年發展的方法，利用心臟病患在經過36次間歇高強度運動訓練前、後血清對心肌細胞行為，超音波影像分析，與心肺運動功能測試；第三年:將健康人與心臟病患的蛋白質體分析，與DNA甲基化的分析。第1年預計招募二十位健康成年男性，接受30分鐘40%、60%或80%的峰值運動強度的一次性運動，實驗結束前、後進行漸進性心肺功能運動測試，並將運動前後取得的血液檢體進行心肌細胞的細胞學實驗；第2年實驗計畫招募四十名心臟病患，將參與者隨機分配到藥物照護組，或間歇高強度運動組(在醫院進行36次3分鐘80%的峰值運動強度，之後進行3分鐘40%的峰值運動強度運動，如此進行5回，共30分鐘)。心臟病患除進行與第一年相同的檢查外，還需在運動前後接受應變超音波的檢查；第3年實驗預計進行前兩年的蛋白質體與基因甲基化的生物資訊分析。We hypothesized that proper exercise will improve hemodynamics. This improvement will activate cardiomyocytes possibly through epigenetic adaptation, which can extend the exercise effects on cardiomyocytes and ultimately improve the survival rate of healthy people or cardiac patients. To confirm this assumption, we will complete the following goals year by year in the future. The first year: we will try to establish the methodology of culturing cardiomyocytes using healthy people's serum before and after acute exercise at different intensities, and to understand the acute physiological adaptation of cardiomyocytes to different exercise intensities; the second year: we will use the method developed in the first year to cultured human cardiomyocyte cell line with cardiac patients' sera before and after 36 times of HIIT. HIIT effects on myocardial cell behavior, strain echocardiographic image analysis, and cardiopulmonary exercise test will then be performed; the third year: serum from healthy people and heart disease patients before and after acute or long-term exercise, respectively, were used to treat cardiomyocytes for 24 hours. The prepared cells underwent proteomic and DNA methylation analysis to find out the protein changed by exercise and the associated epigenetic adaptation. The first year: twenty healthy male adults will be recruited in the first year. An amount of 25 mL of blood will be sampled before interventions and then perform a progressive cardiopulmonary exercise test (CPET) to determine the individual's peak exercise intensity (VO2peak). Subjects then receive a single exercise with VO2peak of 40%, 60%, or 80% for 30 minutes, three times in total. An amount 10 ml blood was sampled (different time points) before, after and two hours after exercise at different intensities. One exercise test and three different exercise intensity experiments are expected in eight weeks. After the end of the experiment, a progressive CPET will be performed again. Blood samples obtained before and after exercise will be analyzed for cell behavior, proteomics, and DNA methylation of cardiomyocytes; the second year: we are going to recruit forty cardiac patients. We will randomly assign participants based on a computer generated program to the medical care (maintaining current medication, recommended home exercise at 60% of VO2peak), or HIIT groups (maintaining the current medication, in-hospital program of 80% of VO2peak for 3 minutes and then 40% of VO2peak for 3 minutes with 5 times for a total of 30 minutes). In addition to the same examinations as in the first year, cardiac patients also undergo strain echocardiography before and after exercise; the third year: experiment is expected to carry out bioinformatic analysis of proteomics and DNA methylation obtained in the first two years.

Keyword(s)

aerobic exercise
cardiomyocytes
proteomics
epigenetics
有氧運動
心肌細胞
蛋白質體
表觀遺傳

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The Aerobic Exercise-Induced Epigenetic Adaptations in Cardiomyocytes(I)

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