Studies on Shellfish Allergy Mechanism Using Systems Biology, RNAi and Cytokine Microarray Techniques (I)

View Statistics Email Alert RSS Feed

Information

Details

Project title

Code/計畫編號

NSC94-2321-B001-025

Translated Name/計畫中文名

以系統生物學、RNA干擾和細胞激素晶片技術探討介貝類引起之食品過敏機制(I)

Project Coordinator/計畫主持人

Tai-Yuan Chen

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

陳水田

Department/Unit

Institute of Biological Chemistry, Academia Sinica

Website

https://www.grb.gov.tw/search/planDetail?id=1121176

Year

2005

Start date/計畫起

01-08-2005

Expected Completion/計畫迄

31-07-2006

Bugetid/研究經費

470千元

ResearchField/研究領域

食品科技(農)

對很多過敏體質的人而言，攝食介貝類會導致全身或局部的過敏現象，嚴重者甚有致死的現象。之前的研究主要探討過敏原與抗體結合位的鑑定和免疫療法進程。在食品過敏預防上，嚴禁接觸或食用過敏原是唯一且較有效的方法。近年來，有些報告針對抗IgE療法、特定DNA序列和細胞激素補償用來治療食品過敏，此類療法因對過敏分子有較好的專一性，故可提高治療效果並減少副作用。本計畫擬以新穎的系統生物學、細胞激素微陣列分析和RNA干擾技術來探討介貝類過敏可能之機制並嘗試增進此類過敏之診斷和治療有效性。本計畫有幾個特定目標，如以下所述： 1. 建立正常人和介貝類過敏患者之血液和尿液蛋白質體學資料庫 2. 介貝類急性過敏之人體血液及老鼠巨大細胞之模式探討 3. 建立蛋白質細胞激素晶片在細胞模式之診斷並評估有效性 4. RNA干擾試驗在細胞模式之治療效果評估 5. 介貝類急性過敏之動物模式探討 6. 確認蛋白質細胞激素晶片在動物模式之診斷有效性 7. RNA干擾試驗在動物模式之治療效果評估 Shellfish play an important role in immediate allergic manifestations including both local and systemic reactions. Actually, food-induced hypersensitivity is the most frequent cause of anaphylaxis seen in emergency department and is responsible for the greater part of anaphylactic deaths in U.S.A. Over the past several years, there have been efforts in allergen identification and quantification to further understand the immunopathologic mechanism of food allergy. To date, the only effective treatment recommended for the prevention of food-induced allergenic reactions is the strict avoidance of the suspicious food. Some promising treatment strategies such as antisense oligonucleotides、small interfering RNA, CpG motif-containing oligodeoxynucleotide, and cytokine gene therapy have been accomplished. We will use novel techniques, systems biology, siRNA, and cytokine microarray, as diagnostic and therapeutic approach for shellfish allergy. In this project, our specific aims are as follows: 1. Establishment of normal and shellfish allergy patients’ sera and urine proteome database 2. Establishment of cell model with immediate shellfish allergy 3. Assessment of shellfish allergy via cytokine microarray under cell model 4. Evaluation on efficiency of siRNA in treatment of shellfish allergy under cell model 5. Establishment of animal model with immediate shellfish allergy 6. Validation of shellfish allergy via cytokine microarray under animal model 7. Evaluation on efficiency of siRNA in treatment of shellfish allergy under animal model

Keyword(s)

介貝類過敏
系統生物學
細胞激素微陣列分析
RNA干擾
Shellfish allergy
Systems biology
Cytokine microarray
RNA interference

DSpace CRIS

Studies on Shellfish Allergy Mechanism Using Systems Biology, RNAi and Cytokine Microarray Techniques (I)

Details

Description