http://scholars.ntou.edu.tw/handle/123456789/17379
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Hung-Yun | en_US |
dc.contributor.author | Wang, Sin-Wen | en_US |
dc.contributor.author | Mao, Ju-Yi | en_US |
dc.contributor.author | Chang, Huan-Tsung | en_US |
dc.contributor.author | Harroun, Scott G. | en_US |
dc.contributor.author | Lin, Han-Jia | en_US |
dc.contributor.author | Huang, Chih-Ching | en_US |
dc.contributor.author | Lai, Jui-Yang | en_US |
dc.date.accessioned | 2021-06-28T02:29:40Z | - |
dc.date.available | 2021-06-28T02:29:40Z | - |
dc.date.issued | 2021-05-01 | - |
dc.identifier.issn | 1385-8947 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/17379 | - |
dc.description.abstract | To prevent visual loss, clinical therapy of severe bacterial keratitis (BK) demands simultaneous remedying of the damage caused by bacterial infection and its induced oxidative stress/inflammation of the cornea. Here, we have developed a one-step method to synthesize carbonized nanogels (CNGs) from biogenic quercetin (Qu) and lysine (Lys) as a bifunctional agent with antibacterial and antioxidant properties for topical BK therapy. These Qu/LysCNGs synthesized by dry heating inherit and amplify the advantages of two natural products, including excellent antioxidant capacity and high positive charge. The Qu/Lys-CNGs display broad-spectrum bacteriostatic effects against non-multidrug-resistant bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, and also against multidrug-resistant bacteria, methicillin-resistant Staphylococcus aureus. Disintegration of peripheral structures of the bacteria caused by Qu/Lys-CNGs is due to their strong and specific interaction with bacterial membranes. In vitro cytotoxicity and hemolysis assays and in vivo corneal biocompatibility evaluations revealed good biocompatibility of Qu/Lys-CNGs as a safe nanomedicine in ophthalmic drop formulation. Furthermore, the Qu/Lys-CNGs can penetrate into the cornea via epithelial tight junction opening, thereby exerting superior antibacterial and antioxidant therapeutic effects against S. aureus-induced keratitis in rabbits. Our results indicate Qu/Lys-CNGs could be an effective bifunctional agent for clinical applications to treat bacterial ocular diseases. | en_US |
dc.publisher | ELSEVIER SCIENCE SA | en_US |
dc.relation.ispartof | CHEMICAL ENGINEERING JOURNAL | en_US |
dc.subject | Bacterial keratitis | en_US |
dc.subject | Topical ocular administration | en_US |
dc.subject | Carbonized nanogels | en_US |
dc.subject | Anti-inflammatory | en_US |
dc.subject | Bacteriostatic agents | en_US |
dc.title | Carbonized nanogels for simultaneous antibacterial and antioxidant treatment of bacterial keratitis | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.cej.2021.128469 | - |
dc.identifier.isi | WOS:000624524100002 | - |
dc.relation.journalvolume | 411 | en_US |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.orcid | 0000-0002-4929-6573 | - |
crisitem.author.orcid | 0000-0002-0363-1129 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
顯示於: | 生命科學暨生物科技學系 |
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